Phospho Stat-3 (pS727) Antibody (GWB-21FC17)

GWB-21FC17

20-203-420051

0.1 ml
$326.00

Description

Clone ID: E121-31

Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 was used as immunogen. The antibody detects Stat3 phosphorylated on Serine 727. It does not cross-react with the phospho-serines of other Stat proteins. Predicted to c Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 was used as immunogen. The antibody detects Stat3 phosphorylated on Serine 727. It does not cross-react with the phospho-serines of other Stat proteins. Dilution: WB: 1:2,000
IHC: 1:50
ICC: 1:100
IP: 1:40. Stat3 is a member of a group of proteins (Signal Transduction and Activators of Transcription) that act as both signal transducers for cytokines and growth factors, as well as transcription factors (1,2). Stats are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus (1). Stat3 is constitutively activated in a number of human tumors (3). Stat3-activated transcription seems to be regulated by serine phosphorylation at Ser727 (4). 1. Simonssona M, Qvarnstro¨ma F, Nymanb J,et al, Low-dose hypersensitive cH2AX response and infrequent apoptosis in epidermis from radiotherapy patients ,Radiotherapy and Oncology 88 (2008) 388-397
[Application: IHC]
2. Wang GF, Mao WM, Zheng S, MicroRNA-183 regulates Ezrin expression in lung cancer cells ,FEBS Letters 582 (2008) 3663-3668
[Application: WB]
3. Lindeman JHN, Abdul-Hussien H, Bockel JH, et al, Clinical Trial of Doxycycline for Matrix Metalloproteinase-9 Inhibition in Patients With an Abdominal Aneurysm Doxycycline Selectively Depletes Aortic Wall Neutrophils and Cytotoxic T Cells ,Circulation 119 (2009) 2209-2216
[Application: WB]
1. Heim, M.H. The Jak-STAT pathway: cytokine signalling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19: 75
2. Kisseleva, T. et al. Signaling through the JAK/STAT pathway, recent advances and future challenges. Gene 285: 1
3. Bromberg, J.F. et al. Stat3 as an Oncogene. Cell 98: 295
4. Wen, Z. et al. Maximal activation of transcription by Statl and Stat3 requires both tyrosine and serine phosphorylation. Cell 82: 241
5. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487

Additional Information

Name Phospho Stat-3 (pS727) Antibody (GWB-21FC17)
Related Product Names Phospho Stat-3 (pS727) Rabbit Monoclonal Antibody STAT3, APRF, Signal transducer and activator of transcription 3, Acute-phase response factorPhospho Stat-3 (pS727)
Swissprot ID P40763
Molecular Weight 92 kDa
Source Rabbit
Application WB, IHC, ICC, IP
Clonality Monoclonal
Reactivity Human, Rat
Storage Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Datasheets/Manuals Printable datasheet for GWB-21FC17
Intended Use Research Use Only