Human TNF RII-FcA Chimera (GWB-549497)



0.02 mg


Source: A DNA sequence encoding the signal peptide and extracellular domain of human TNF Receptor II (TNFRII) (aa 1-253) was fused to the Fc region of human IgG1 (aa 93-330). The chimeric protein was expressed in modified human 293 cells.

Molecular Mass: TNF RII-FcB Chimera migrates as a band between 55 and 80 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified TNF RII-Fc Chimera that has a predicted molecular mass of 51.8 kDa.

PI: TNF RII-FcB Chimera separates into a number of glycoforms with a pI between 4.5 and 7.0 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified TNF RII-Fc Chimera that has a predicted pI of 7.9. Theoretical Sequence: LPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPTRSMAPG AVHLPQPVST RSQHTQPTPE PSTAPSTSFL LPMGPSPPAE GIPKVDKKVE PKSCDKTHTC PPCPA Activity: The ND50 of TNF RII-FcB Chimera is typically 20-50 ng/ml as measured by its ability to neutralize TNF-a mediated cytoxicity in murine WEHI 164 cells in the presence of actinomycin D.

% Carbohydrate: purified TNF RII-FcB Chimera consists of 5-35% carbohydrate by weight.

Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Tumor necrosis factor alpha (TNF-alpha ; TNF alpha) and the structurally related lymphotoxin-alpha (LT-a) are pro-inflammatory cytokines produced by a variety of immune and non-immune cells in response to multiple stimuli. These stimuli can include bacteria and bacterial products such as LPS, viral infections, T-cell receptor activation and cross-linking of surface immunoglobulin (Ig) on B cells. Expression of TNF-a has been detected in macrophages/monocytes, B-cells, T-cells, basophils, eosinophils, glial cells and mast cells. The effects of TNF-alpha and LT-a are mediated through the TNF receptors, TNF RI and TNF RII. These receptors are type I transmembrane glycoproteins belonging to the TNF receptor superfamily, which is defined by the presence of repeating units of cysteine clusters. TNF RI is present on virtually all cell types except for red blood cells. For a review of the mechanisms that regulate TNF RII expression refer to Carpentier I, et al (2004) Curr Med Chem. 11(16): 2205-12.

Function: Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Subunit: Binds to TRAF2.

Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tumor necrosis factor-binding protein 2: Secreted.

Ptm: Phosphorylated; mainly on serine residues and with a very low level on threonine residues.

Ptm: A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.

Pharmaceutical: Available under the name Enbrel (Immunex and Wyeth-Ayerst). Used to treat moderate to severe rheumatoid arthritis (RA). Enbrel consist of the extracellular ligand-binding portion of TNFRSF1B linked to an immunoglobulin Fc chain. It binds to TNF-alpha and blocks its interactions with receptors.

Similarity: Contains 4 TNFR-Cys repeats.

Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways.

Additional Information

Name Human TNF RII-FcA Chimera (GWB-549497)
Related Product Names human cell expressed TNF RII-FcA Chimera Human TNF RII-FcA ChimeraTNF RII
Swissprot ID P20333
NCBI Acc Number NP_001057.1
Molecular Weight 48291
Purity >95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
GI Number 4507577
Format When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reactivity Human
Storage Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 4C is recommended, with longer-term storage in aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
Datasheets/Manuals Printable datasheet for GWB-549497
Intended Use Research Use Only