TNF alpha: Human Recombinant E Coli (GWB-56E953)



1 mg


TNF alpha is a potent pleiotrophic cytokine, produced by white blood cells, endothelium and several other normaland tumour cells in response to a wide variety of stimuli.TNF alpha is involved in the pathophysiological processes of several chronic and acute diseases including the induction of apoptosis and up regulation ofinflammation.
RANDOX recombinant TNF alpha comprises a 157amino acid fragment (77-233) corresponding to the mature soluble TNF alpha chain protein. TNF alpha is expressed in E. coli with an amino-terminal hexahistidinetag. This product is for research use only and is not intended for diagnostic or therapeutic use.

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Subunit: Homotrimer.

Subcellular Location: Cell membrane; Single-pass type II membrane protein.

Subcellular Location: Tumor necrosis factor, soluble form: Secreted.

Ptm: The soluble form derives from the membrane form by proteolytic processing.

Ptm: The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.

Ptm: O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Disease: Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition.

Disease: Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis.

Disease: Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.

Similarity: Belongs to the tumor necrosis factor family. 1: Carswell, E et al., (1975) Proc. Natl. Acad. Sci U.S.A. 72 (9): 3666-3670.

Additional Information

Name TNF alpha: Human Recombinant E Coli (GWB-56E953)
Availability Discontinued
Related Product Names Tumour necrosis factor alpha; TNFA; TNFá; Tumournecrosis factor ligand superfamily 2; TNFSF2; Cachectin;Cachexin; APC1 protein; DIF; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin TNFA; TNFSF2TNF alpha: Human, Recombinant,
Swissprot ID P01375
NCBI Acc Number NP_000585.2
Molecular Weight 25644
Purity >95% by SDS-PAGE
Source E Coli
Application SDS-Page
Format Liquid
Datasheets/Manuals Printable datasheet for GWB-56E953
Intended Use Research Use Only