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0.1 mg
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Use: The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made accrording to the amouonts TNF-alpha to be inactivated,. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added Applications: The antibody can be used for inhibition of biological activity. Furthermore the antibody is useful for immuno histology on both frozen and paraffin sections. flow cytometry. immuno assay and Western blotting. Tumor Necrosis Factor alpha (TNF-alpha) is a cytokine which has diverse immunomodulatory. anti tumor and toxic effects. TNF-alpha has been detected in diverse inflammatory status and appears to be a critical mediator in the lethality of septic shock. Furthermore. TNF-alpha has also been found in inflammatory foci such as synovial effusions in rheumatoid arthritis. systemic circulation in septic shock. parasitemia and rejection of renal transplants. The antibody reacts with both rat and mouse natural and recombinant TNF-alpha and recognizes membrane and receptor bound TNF-alpha. The antibody shows neutralizing activity.

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Subunit: Homotrimer.

Subcellular Location: Cell membrane; Single-pass type II membrane protein.

Subcellular Location: Tumor necrosis factor, soluble form: Secreted.

Ptm: The soluble form derives from the membrane form by proteolytic processing.

Ptm: The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.

Ptm: O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.

Disease: Cachexia accompanies a variety of diseases, including cancer and infection, and is characterized by general ill health and malnutrition.

Disease: Genetic variations in TNF are associated with susceptibility to psoriatic arthritis [MIM:607507]. Psoriasis is a chronic inflammatory dermatosis that affects approximately 2% of the population. It is characterized by red, scaly skin lesions that are usually found on the scalp, elbows, and knees, and may be associated with severe arthritis. Psoriatic arthritis has been defined as an inflammatory arthritis usually without any rheumatoid factor in serum (seronegative arthritis) associated with psoriasis.

Disease: Genetic variations in TNF are associated with susceptibility to hepatitis B virus infection (HBV infection) [MIM:610424]. Approximately one third of all cases of cirrhosis and half of all cases of hepatocellular carcinoma can be attributed to chronic HBV infection. HBV infection may result in subclinical or asymptomatic infection, acute self-limited hepatitis, or fulminant hepatitis requiring liver transplantation.

Similarity: Belongs to the tumor necrosis factor family.

Additional Information

Related Product Names POLYCLONAL ANTIBODY TO MOUSE TUMOR NECROSIS FACTOR-alpha CROSS REACTIVE WITH RAT TNF-alpha; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin TNFA; TNFSF2TUMOR NECROSIS FACTOR-alpha CROSS REACTIVE WITH RAT TNF-alphaTNF
Swissprot ID P01375
NCBI Acc Number NP_000585.2
Molecular Weight 25644
Source Rabbit IgG
Clonality Polyclonal
Format 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS. containing 0.02% sodium azide and 0.1% bovine serum albumin.
Stability Stable for 1 year
Reactivity Mouse
Storage Product should be stored at 4 degree C
Datasheets/Manuals Printable datasheet for GWB-77B2CF
Intended Use Research Use Only