TNF-RECEPTOR I (p55/p60) Antibody (GWB-59354B)



0.1 mg
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Clone: HM104 Applications: The monoclonal antibody HM104 can be used for flow cytometry. Furthermore the monoclonal antibody HM104 is useful for immuno assays. immunoprecipitation and immuno histology on frozen sections. The reactivity of the antibody with soluble TNF-Receptor is inhibited by high concentrations of both mouse and human TNF-alpha. The monoclonal antibody HM104 reacts with the extra-cellular part of the mouse TNF-RI and with the soluble receptor. TNF-RI is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. Induction of cytotoxicity and other most functions are mediated largely via TNF-RI.

Function: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase.

Subunit: Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein.

Subcellular Location: Cell membrane; Single-pass type I membrane protein. Secreted.

Domain: The domain that induces A-SMASE is probably identical to the death domain. The N-SMASE activation domain (NSD) is both necessary and sufficient for activation of N-SMASE.

Domain: Both the cytoplasmic membrane-proximal region and the C-terminal region containing the death domain are involved in the interaction with TRPC4AP (By similarity).

Ptm: The soluble form is produced from the membrane form by proteolytic processing.

Disease: Defects in TNFRSF1A are the cause of familial hibernian fever (FHF) [MIM:142680]; also known as tumor necrosis factor receptor-associated periodic syndrome (TRAPS). FHF is an autosomal dominant disease characterized by recurrent fever, abdominal pain, localized tender skin lesions and myalgia.

Similarity: Contains 1 death domain.

Similarity: Contains 4 TNFR-Cys repeats. [1] Gray,P.W., Barrett,K., Chantry,D., Turner,M. and Feldmann,M., et al.
Cloning of human tumor necrosis factor (TNF) receptor cDNA and expression of recombinant soluble TNF-binding protein
[2] Schall T.J., Lewis M., Koller K.J., Lee A., Rice G.C., Wong G.H.W., Getanaga T., Granger G.A., Lentz R., Raab H., et al.
Molecular cloning and expression of a receptor for human tumor necrosis factor.
[3] Loetscher H., Pan Y.-C.E., Lahm H.-W., Gentz R., Brockhaus M., Tabuchi H., Lesslauer W.
Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor.
[4] Nophar Y., Kemper O., Brakebusch C., Engelmann H., Zwang R., Aderka D., Holtmann H., Wallach D.
Soluble forms of tumor necrosis factor receptors (TNF-Rs). The cDNA for the type I TNF-R, cloned using amino acid sequence data of its soluble form, encodes both the cell surface and a soluble form of the receptor.
[5] Himmler A., Maurer-Fogy I., Kroenke M., Scheurich P., Pfizenmaier K., Lantz M., Olsson I., Hauptmann R., Stratowa C., Adolf G.R., et al.
Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein.
[6] Gray P.W., Barrett K., Chantry D., Turner M., Feldman M.
Cloning of human tumor necrosis factor (TNF) receptor cDNA and expression of recombinant soluble TNF-binding protein.
[7] Fuchs P., Strehl S., Dworzak M., Himmler A., Ambros P.F.
Structure of the human TNF receptor 1 (p60) gene (TNFR1) and localization to chromosome 12p13.
[8] Rieder M.J., Armel T.Z., Carrington D.P., Ozuna M., Kuldanek S.A., Rajkumar N.R., Toth E.J., Yi Q., Nickerson D.A.
SeattleSNPs. NHLBI HL66682 program for genomic applications, UW-FHCRC, Seattle, WA (URL:
[9] Engelmann H., Novick D., Wallach D.
Two tumor necrosis factor-binding proteins purified from human urine. Evidence for immunological cross-reactivity with cell surface tumor necrosis factor receptors.
[10] Zhu N., Khoshnan A., Schneider R., Matsumoto M., Dennert G., Ware C.F., Lai M.M.C.
Hepatitis C virus core protein binds to the cytoplasmic domain of tumor necrosis factor (TNF) receptor 1 and enhances TNF-induced apoptosis.

Additional Information

Name TNF-RECEPTOR I (p55/p60) Antibody (GWB-59354B)
Related Product Names MONOCLONAL ANTIBODY TO MOUSE TNF-RECEPTOR I (p55/p60); p60; TNF-R1; TNF-RI; TNFR-I; p55; CD120a antigen TNFAR; TNFR1TNF-RECEPTOR I (p55/p60)TNFRSF1A
Swissprot ID P19438
NCBI Acc Number AAA36756.1
Molecular Weight 50495
Source Rat IgG2a
GI Number 339760
Clonality Monoclonal
Format 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS. containing 0.02% sodium azide and 0.1% bovine serum albumin.
Stability Stable for 1 year
Reactivity Mouse
Sequence Length 453
Storage Product should be stored at 4 degree C
Datasheets/Manuals Printable datasheet for GWB-59354B
Intended Use Research Use Only