CD120b. TNF-RII (p75/p80) Antibody (GWB-CF3348)



0.1 mg
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Clone: MR2-1 Applications: The monoclonal antibody MR2-1 is an agonistic antibody (after cross-linking) and receptor modulating antibody useful for cell culture experiments. flow cytometry and immunohistology on frozen sections. Furthermore the monoclonal antibody MR2-1 is useful for immunoprecipitation. Western blotting and immuno assays. The reactivity of the antibody MR2-1 with cell-bound TNF-Receptor is minimally inhibited by high concentrations of TNF-alpha . The antibody MR2-1 reacts with the extra-cellular part of the TNF-RII. It also reacts with the soluble receptor. TNF-RII is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-RII molecule is shown to be responsible for stimulation of activated T-lymphocytes by TNF-alpha. The antibody cross reacts with rhesus and cynomolgus natural TNF-RII.

Function: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Association to the receptor is also mediated by the interaction with TRADD. Mediates activation of NF-kappa-B and JNK and is involved in apoptosis. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. Seems to be involved in IL-15 signaling.

Subunit: Homotrimer (Probable). Heteromer with TRAF1. Binds to TNFRSF1B/TNFR2, TNFRSF4 and TNFRSF5/CD40. Associates with CD27, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13B/TACI. TNFRSF14, TNFRSF16/NGFR, TNFRSF17/BCMA, TNFRSF18/AITR, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1, IL15RA, TANK/ITRAF, RIPK2, TNIK, MAP3K14, MAP3K5, MAP3K1, MAP4K2, CDK9, CSK, and TRAF-interacting protein TRAIP and TRAF and TNF receptor associated protein TTRAP. Interacts with TNFAIP3 and TRPC4AP. Interacts with PEG3 (By similarity). Binds to TRADD. Interacts with BIRC2 and BIRC3 N-terminus. Interacts with CYLD and TBK1. Interacts with MAVS/IPS1. Interacts with CASP8AP2 and USP48. Interacts with DAB2IP. Interacts wtih NFATC2IP (By similarity).

Subcellular Location: Cytoplasm.

Domain: The coiled coil domain mediates homo- and hetero-oligomerization.

Domain: The MATH/TRAF domain binds to receptor cytoplasmic domains.

Ptm: Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation. Not ubiquitinated by SIAH1.

Similarity: Contains 1 MATH domain.

Similarity: Contains 1 RING-type zinc finger.

Similarity: Contains 2 TRAF-type zinc fingers.

Summary: The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [1] Varfolomeev,E., Wayson,S.M., Dixit,V.M., Fairbrother,W.J. and Vucic,D.
The inhibitor of apoptosis protein fusion c-IAP2.MALT1 stimulates NF-kappaB activation independently of TRAF1 AND TRAF2
[2] Wu,Y., Fan,Y., Xue,B., Luo,L., Shen,J., Zhang,S., Jiang,Y. and Yin,Z.
Human glutathione S-transferase P1-1 interacts with TRAF2 and regulates TRAF2-ASK1 signals
[3] Wang,J., Li,C., Liu,Y., Mei,W., Yu,S., Liu,C., Zhang,L., Cao,X., Kimberly,R.P., Grizzle,W. and Zhang,H.G.
JAB1 determines the response of rheumatoid arthritis synovial fibroblasts to tumor necrosis factor-alpha
[4] Lim,J., Hao,T., Shaw,C., Patel,A.J., Szabo,G., Rual,J.F., Fisk,C.J., Li,N., Smolyar,A., Hill,D.E., Barabasi,A.L., Vidal,M., et al.
A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration
[5] Uemura,N., Kajino,T., Sanjo,H., Sato,S., Akira,S., Matsumoto,K. and Ninomiya-Tsuji,J.
TAK1 is a component of the Epstein-Barr virus LMP1 complex and is essential for activation of JNK but not of NF-kappaB
[6] Song H.Y., Donner D.B.
Association of a RING finger protein with the cytoplasmic domain of the human type-2 tumour necrosis factor receptor.
[7] Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., et al.
Complete sequencing and characterization of 21,243 full-length human cDNAs.
[8] Rieder M.J., Daniels R.L., da Ponte S.H., Hastings N.C., Ahearn M.O., Rajkumar N., Yi Q., Nickerson D.A.
SeattleSNPs. NHLBI HL66682 program for genomic applications, UW-FHCRC, Seattle, WA (URL:
[9] Rothe M., Wong S.C., Henzel W.J., Goeddel D.V.
A novel family of putative signal transducers associated with the cytoplasmic domain of the 75 kDa tumor necrosis factor receptor.
[10] Pomerantz J.L., Baltimore D.
NF-kB activation by a signaling complex containing TRAF2, TANK, and TBK1, a novel IKK-related kinase.

Additional Information

Name CD120b. TNF-RII (p75/p80) Antibody (GWB-CF3348)
Availability In Stock
Related Product Names MONOCLONAL ANTIBODY TO HUMAN CD120b. TNF-RII (p75/p80); Tumor necrosis factor type 2 receptor-associated protein 3 TRAP3CD120b. TNF-RII (p75/p80)TRAF2
Swissprot ID Q12933
NCBI Acc Number NP_066961.2
Molecular Weight 55859
Source Mouse IgG1
GI Number 22027612
Clonality Monoclonal
Format 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS. containing 0.02% sodium azide and 0.1% bovine serum albumin.
Stability Stable for 1 year
Reactivity Human
Sequence Length 501
Storage Product should be stored at 4 degree C
Datasheets/Manuals Printable datasheet for GWB-CF3348
Intended Use Research Use Only