Cdk2 (C-term) Antibody (GWB-3A107F)



0.1 ml


Clone ID: E304

Immunogen: A synthetic peptide corresponding to residues in C-terminus of human Cdk2 was used as immunogen. Predicted to cross-react with mouse and rat, based on sequence homology. Specificity: A synthetic peptide corresponding to residues in C-terminus of human Cdk2 was used as immunogen. Dilution: WB: 1:1000 - 5000
ICC: 1:100
IP: 1:40. Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 34-kDa nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4). 1. Waldow T, Witt W, UlmerA, et al, Preconditioning by inhaled nitric oxide prevents hyperoxic and ischemia/reperfusion injury in rat lungs ,Pulmonary Pharmacology & Therapeutics 21 (2008) 418-429
[Application: WB]
2. Jeon S, Kim NH, Kim JY, et al, Stem cell factor induces ERM proteins phosphorylation through PI3K activation to mediate melanocyte proliferation and migration ,Pigment Cell Melanoma Res. 22 (2009) 77-85
[Application: WB]
1. Morgan, D.O. Principles of CDK regulation. Nature 374: 131
2. Elledge, S.J., et al. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle. Proc. Natl. Acad. Sci. USA 89: 2907
3. Gu, Y., et al. Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15. EMBO J. 11: 3995
4. Fesquet, D., et al. The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues. EMBO J. 12: 3111
5. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487

Additional Information

Name Cdk2 (C-term) Antibody (GWB-3A107F)
Related Product Names Cdk2 (C-term) Rabbit Monoclonal Antibody CDK2, Cell division protein kinase 2, p33 protein kinaseCdk2 (C-term)
Swissprot ID P24941
Molecular Weight 33 kDa
Source Rabbit
Application WB, ICC, IP
Clonality Monoclonal
Reactivity Human, Mouse
Datasheets/Manuals Printable datasheet for GWB-3A107F
Storage Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Intended Use Research Use Only