cdk7/CAK Antibody (GWB-477DB1)

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Similarity: Contains 1 protein kinase domain.

Buffer: Lyophilized from PBS, pH 7.4 containing 0.5% BSA

Function: Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Additional Information

Name cdk7/CAK Antibody (GWB-477DB1)
Related Product Names MAb to cdk7/CAK; EC; EC; CDK-activating kinase; CAK; CAK1; TFIIH basal transcription factor complex kinase subunit; 39 kDa protein kinase; P39 Mo15; STK1 MO15cdk7/CAKCDK7
Gene id 1022
Alias Symbols Monoclonal Antibody to Human cdk7/Cyclin Activating Kinase (CAK)
Swissprot ID P50613
Protein Name Cyclin-dependent kinase 7
Protein Accession Num NP_001790.1
Clone MO-1.1
Application Info Immunohistochemistry of frozen sections (1:10 - 1:20).
Immunoprecipitation of active kinase complex
Cell sorting (FACS analysis)
Each laboratory should determine an optimum working titer for use in its part
Host Mouse. Source: Cell Culture
Isotype IgG2b
NCBI Acc Number NP_001790.1
Product Format Purified, Lyophilized.
Reconstitute in 1ml distilled water.
Specificity cdk7/Cyclin Activating Kinase (CAK)
Recognizes the key cell cycle regulator cdk7, which is essential to activate the cdk2 kinase in G1 phase and the cdc2 kinase in G2/M phase. The antibody is useful for cell cycles analysis, cell proliferation studies
Purity Protein A chromatography.
Application Flow Cytometry (FACS), IF, IHC-F, WB
Application Data Immunogen: Recombinant C-terminal 221 amino acid fragment of human wild-type cdk7/CAK (cyclin activating kinase).
Affinity Constant: Not determined.
Clonality Monoclonal
Format Purified, Lyophilized. Reconstitute in 1ml distilled water.
Reconstitution and Storage Store lyophilized product at 2 to 8C. After reconstitution, short term (up to one year) store at 2 to 8C. Long term, aliquot and store at -20C. Avoid multiple freeze/thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-477DB1
Storage Store lyophilized product at 2-8 C. After reconstitution, short term (up to one year) store at 2-8 C. Long term, aliquot and store at -20 C. Avoid multiple freeze/thaw cycles.
Lead Time Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Intended Use Research Use Only
:: Buffer: Lyophilized from PBS, pH 7.4 containing 0.5% BSA
Preservative: 0.09% Sodium azide
Lyophilized: Yes
Warnings: This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes