cyclin-dependent kinase 7 (MO15 homolog Xenopus laevis cdk-activating kinase) (CDK7) Antibody (GWB-928736)



1 ml


Clone: 2141C4a

IGG Subclass: IgG1 Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.

Sterility: Filtered through a 0.22 um membrane. Function: Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between two subsequent phases in the cell cycle. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Catalytic Activity: ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Enzyme Regulation: Inactivated by phosphorylation.

Subunit: Associates primarily with cyclin H and MAT1 to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.

Subcellular Location: Nucleus.

Tissue Specificity: Ubiquitous.

Ptm: Phosphorylation of Ser-164 during mitosis inactivates the enzyme.

Ptm: Phosphorylation of Thr-170 is required for activity.

Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Similarity: Contains 1 protein kinase domain.

Summary: The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle. [1] Larochelle,S., Merrick,K.A., Terret,M.E., Wohlbold,L., Barboza,N.M., Zhang,C., Shokat,K.M., Jallepalli,P.V. and, et al.
Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells
[2] Wissing,J., Jansch,L., Nimtz,M., Dieterich,G., Hornberger,R., Keri,G., Wehland,J. and Daub,H.
Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry
[3] Mehrle,A., Rosenfelder,H., Schupp,I., del Val,C., Arlt,D., Hahne,F., Bechtel,S., Simpson,J., Hofmann,O., Hide,W.,, et al.
The LIFEdb database in 2006
[4] Larochelle,S., Batliner,J., Gamble,M.J., Barboza,N.M., Kraybill,B.C., Blethrow,J.D., Shokat,K.M. and Fisher,R.P.
Dichotomous but stringent substrate selection by the dual-function Cdk7 complex revealed by chemical genetics
[5] Jang,M.K., Mochizuki,K., Zhou,M., Jeong,H.S., Brady,J.N. and Ozato,K.
The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription
[6] Tassan J.-P., Schultz S.J., Bartek J., Nigg E.A.
Cell cycle analysis of the activity, subcellular localization, and subunit composition of human CAK (CDK-activating kinase).
[7] Levedakou E.N., He M., Baptist E.W., Craven R.J., Cance W.G., Welcsh P.L., Simmons A., Naylor S.L., Leach R.J., Lewis T.B., et al.
Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern.
[8] Darbon J.-M., Devault A., Taviaux S., Fesquet D., Martinez A.M., Galas S., Cavadore J.-C., Doree M., Blanchard J.-M.
Cloning, expression and subcellular localization of the human homolog of p40MO15 catalytic subunit of cdk-activating kinase.
[9] Wu L., Yee A., Liu L., Carbonaro-Hall D., Venkatesan N., Tolo T., Hall F.L.
Molecular cloning of the human CAK1 gene encoding a cyclin-dependent kinase-activating kinase.
[10] Kobelt D., Karn T., Hock B., Holtrich U., Braeuninger A., Wolf G., Strebhardt K., Ruebsamen-Waigmann H.
Human and Xenopus MO15 mRNA are highly conserved but show different patterns of expression in adult tissues.

Additional Information

Name cyclin-dependent kinase 7 (MO15 homolog Xenopus laevis cdk-activating kinase) (CDK7) Antibody (GWB-928736)
Related Product Names Mouse monoclonal anti-human CDK7 antibody; EC; EC; CDK-activating kinase; CAK; TFIIH basal transcription factor complex kinase subunit; 39 kDa protein kinase; P39 Mo15; STK1; CAK1 CAK1; STK1;CDKN7; p39MO15cyclin-dependent kinase 7 (MO1
Swissprot ID P50613
NCBI Acc Number NM_001799.2
Molecular Weight 0.5
Source Mouse
Application DB, Flow Cytometry (FACS), ICC, IP , WB
GI Number 16950659
Clonality Monoclonal
Format Mouse monoclonal anti-human CDK7 antibody in PBS (3.0 mM KCl, 1.5 mM K2HPO4, 140 mM NaCl, 8.0 mM Na2HPO4 (pH 7.4)) containing 1% bovine serum albumin (BSA) and 0.05% sodium azide (NaN3).
Reactivity Human
Datasheets/Manuals Printable datasheet for GWB-928736
Storage Store at 2-8 C for up to one year. We recommend storing at –20 C for long-term storage. Avoid repeat freezing and thawing cycles.
Intended Use Research Use Only