p27 (Kip1) (pS10) Phospho Antibody (GWB-8BB812)

GWB-8BB812

20-203-420733

0.1 ml
$326.00

Description

Clone ID: EP233(2)Y

Immunogen: A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10. Specificity: A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10. Dilution: WB: 1:1,000 - 10,000
IHC: 1:100
IP: 1:30. p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3). 1. Iwahori S, Murata T, Kudoh A, et al, Phosphorylation of p27Kip1 by Epstein-Barr Virus Protein Kinase Induces Its Degradation through SCFSkp2 Ubiquitin Ligase Actions during Viral Lytic Replication ,J. Biol. Chem. 284 (2009) 18923 - 18931
[Application: WB]
1. Polyak K., et al; Cell 78:59-66,1994.
2. Carrano,A.C., et al. Nat. Cell Biol., 1, 193-199
3. Rodier,G.,et al. EMBO J., 20, 6672-6682, 2001
4. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487

Additional Information

Name p27 (Kip1) (pS10) Phospho Antibody (GWB-8BB812)
Related Product Names p27 (Kip1) (pS10) Phospho Rabbit Monoclonal Antibody CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27;p27Kip1p27 (Kip1) (pS10) Phospho
Swissprot ID P46527
Molecular Weight 27 kDa
Source Rabbit
Application WB, IHC, IP
Clonality Monoclonal
Reactivity Human, Mouse, Rat
Datasheets/Manuals Printable datasheet for GWB-8BB812
Storage Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Intended Use Research Use Only