p27kip1 Antibody (GWB-9BFA1A)

GWB-9BFA1A

20-203-420217

0.1 ml
$294.00

Description

Clone ID: Y236

Immunogen: A synthetic peptide corresponding to residues in the C-term of human p27(Kip1) was used as immunogen Specificity: A synthetic peptide corresponding to residues in the C-term of human p27(Kip1) was used as immunogen Dilution: WB: 1:500 - 1,000
IHC: 1:100-250
ICC: 1:100-250
FC: 1:40
IP: 1:50. p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3). 1. Polyak K., Lee M.-H., Erdjument-Bromage H., Koff A., Roberts J.M., Tempst P., Massague J.; Cell 78:59-66 (1994).
2. Carrano,A.C., Eytan,E., Hershko,A. and Pagano,M. (1999) SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat. Cell Biol., 1, 193-199
3. Rodier,G., Montagnoli,A., Di Marcotullio,L., Coulombe,P., Draetta,G.F., Pagano,M. and Meloche,S. (2001). EMBO J., 20, 6672-6682
4. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487

Additional Information

Name p27kip1 Antibody (GWB-9BFA1A)
Related Product Names p27kip1 Rabbit Monoclonal Antibody CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27;p27Kip1p27kip1
Swissprot ID P46527
Molecular Weight 27 kDa
Source Rabbit
Application WB, IHC, ICC, FC IP
Clonality Monoclonal
Reactivity Human, Rat
Datasheets/Manuals Printable datasheet for GWB-9BFA1A
Storage Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Intended Use Research Use Only