Anti- p27Kip1 (Ab-10) Antibody Antibody (GWB-37FF7C)

GWB-37FF7C

18-785-210327

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Description

Binding Site: around Ser10

Form of Antibody: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Immunogen: The antiserum was produced against syntheseized non-phosphopeptide derived from human p27Kip1 around the phosphorylation site of serine 10 (N-G-Sp-P-S).

Purification: The antibody was affinity-chromatography using epitope-specific immunogen. WB Dilution: 500~1000

IHC Dilution: 50~100 Specificity/Sensitivity: p27Kip1 (Ab-10) antibody detects endogenous levels of total p27Kip1 protein. Function: Involved in G1 arrest. May mediate TGF beta-induced G1 arrest. Binds to and inhibits complexes formed by cyclin E-CDK2, cyclin A-CDK2, and cyclin D1-CDK4. Interaction with nucleoporin NUP50 is required for nuclear import and for degradation of phosphorylated p27Kip1 after nuclear import (By similarity).

Subunit: Interacts with NUP50 and with UHMK1. Interacts with COPS5 subunit of COP9 signalosome complex, leading to its subsequent degradation (By similarity). Interacts with SPDYA and is found in a complex with both SPDYA and CDK2.

Subcellular Location: Nucleus (By similarity). Cytoplasm (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. Upon cell cycle progression, mostly cytoplasmic (By similarity).

Tissue Specificity: Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

Domain: A peptide sequence containing only AA 28-79 retains substantial KIP1 cyclin A/CDK2 inhibitory activity.

Ptm: Phosphorylation on Ser-10 leads to nuclear export to the cytoplasm and promotes cell cycle progression (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.

Disease: Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.

Similarity: Belongs to the CDI family. [1] Polyak K., Lee M.-H., Erdjument-Bromage H., Koff A., Roberts J.M., Tempst P., Massague J.
Cloning of p27Kip1, a cyclin-dependent kinase inhibitor and a potential mediator of extracellular antimitogenic signals.
[2] Pietenpol J.A., Bohlander S.K., Sato Y., Papadopoulos N., Liu B., Friedman C., Trask B.J., Roberts J.M., Kinzler K.W., Rowley J.D., et al.
Assignment of the human p27Kip1 gene to 12p13 and its analysis in leukemias.
[3] Rieder M.J., Braun A.C., Montoya M.A., Chung M.-W., Nguyen C.P., Nguyen D.A., Livingston R.J., Poel C.L., Robertson P.D., Schackwitz W.S., et al.
NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).
[4] Boehm M., Yoshimoto T., Crook M.F., Nallamshetty S., True A., Nabel G.J., Nabel E.G.
A growth factor-dependent nuclear kinase phosphorylates p27(Kip1) and regulates cell cycle progression.
[5] Porter L.A., Kong-Beltran M., Donoghue D.J.
Spy1 interacts with p27Kip1 to allow G1/S progression.
[6] Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
[7] Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., et al.
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.
[8] Russo A.A., Jeffrey P.D., Patten A.K., Massague J., Pavletich N.P.
Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor bound to the cyclin A-Cdk2 complex.
[9] Pellegata N.S., Quintanilla-Martinez L., Siggelkow H., Samson E., Bink K., Hoefler H., Fend F., Graw J., Atkinson M.J.
Germ-line mutations in p27(Kip1) cause a multiple endocrine neoplasia syndrome in rats and humans.

Additional Information

Name Anti- p27Kip1 (Ab-10) Antibody Antibody (GWB-37FF7C)
Related Product Names Cyclin-dependent kinase inhibitor p27; p27Kip1 KIP1p27Kip1 (Ab-10) AntibodyP27KIP1
Swissprot ID P46527
Molecular Weight 22073
Purity Affinity Purified
Source Rabbit
Application IHC, WB
Clonality Polyclonal
Format In PBS, 0.02% sodium azide and 50% glycerol.
Stability 1 year at -20C
Reactivity Human, Mouse, Rat
Datasheets/Manuals Printable datasheet for GWB-37FF7C
Storage 4C for short term (weeks) and -20C for long term. Avoid frequent freeze and thaw.
Intended Use Research Use Only