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PLK1, Active Human Recombinant Protein

GWB-55E483

10µg
$348.50
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Description

Description: Human Plk1 (GenBank Accession No. NM_005030), full length with C-terminal HIS6 tag, MW=68 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 35 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.1 mM ATP. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Stability: >6 months at -80°C. References: 1. Fabbro, M. et al., Dev. Cell 9 (4), 477-488. (2005) 2. Liu, X., et al., Mol. Cell. Biol. 25, 4993-5010 (2005)

Host: baculovirus

Tag: C-terminal His tag

GI Num: NM_005030

MW: 68 kDa

Format: aqueous buffer solution

Formula: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole

Scientific Category: Kinase/Serine-Threonine

Species: human

Additional Information

Name PLK1, Active Human Recombinant Protein
Related Product Names PLK1, Active Human Recombinant Protein
Molecular Weight 68 kDa
Purity 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Source baculovirus
GI Number NM_005030
Format Aqueous Buffer Solution
Reactivity Human
Storage -80°C
Datasheets/Manuals Printable datasheet for GWB-55E483
Intended Use Research Use Only