Anti- Polo-like kinase kinase 1 Antibody (GWB-6352E0)

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100 ul


Similarity: Belongs to the Ser/Thr protein kinase family.

Additional Information

Name Anti- Polo-like kinase kinase 1 Antibody (GWB-6352E0)
Related Product Names Polo-like kinase kinase 1xPlkk1
Gene Symbol PLK1
Gene id 380481
Swissprot ID P70032
Protein Name Serine/threonine-protein kinase PLK1
Description of Target Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to
Protein Accession Num NP_001080788.1
Application Info The antibody has been directly tested for reactivity in Western blots with Xenopus tissue. It is anticipated that the antibody will react with human, mouse, rat and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Host Rabbit
Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser482,486,490 of Xenopus polo-like kinase kinase (PLKK).
Replacement This antibody may replace item sc-111085 from Santa Cruz Biotechnology.
Homology Human; Mouse; Rat; Zebrafish
NCBI Acc Number AAC95157.1
Product Format Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Molecular Weight ~120kDa
Purification Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Specificity Specific for the ~120k PLKK protein phosphorylated at Ser482,486,490.
Application WB
GI Number 3983166
Clonality Polyclonal
Stability 6-12 months at -20 degree C.
Reconstitution and Storage For long term storage -20C is recommended. Stable at -20C for at least 1 year.
Reactivity Xenopus
Sequence Length 950
Datasheets/Manuals Printable datasheet for GWB-6352E0
Storage 4 degree C for short term (weeks) and -20 degree C for long term. Avoid frequent freeze and thaw.
Species Reactivity Human, Mouse, Rat, Zebrafish
Lead Time Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Intended Use Research Use Only
Key Reference Erikson E, Haystead TAJ, Qian, Y-W, Maller JL (2004) A feedback loop in the polo-like kinase activation pathway. J Biol Chem 279:32219-32224.
Kumagai A, Dunphy WG (1996) Purification and molecular cloning of Plx1, a cdc25-regulatory kinase from Xenopus egg extracts. Science 273:1377-1380.
Liu J, Lewellyn AL, Chen LG, Maller JL (2004) The polo box is required for multiple functions of Plx1 in mitosis. J Biol Chem 279:21367-21373.
van Vugt, MATM, van de Weerdt, BCM, Vader G, Janssen H, Calafat J, Klompmaker R, Wolthuis RMF, Medema RH (2004) Polo-like kinase-1 is required for bipolar spindle formation but is dispensable for anaphase promoting complex/cdc20 activation and initiation of cytokinesis. J Biol Chem 279:36841-36854.
Eleanor Erikson, Timothy A. J. Haystead, Yue-Wei Qian, and James L. Maller (2004) A Feedback Loop in the Polo-like Kinase Activation Pathway. J. Biol. Chem., Jul 2004; 279: 32219 - 32224
Additional Information Packaging :
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.