PI3-kinase p110 subunit alpha Antibody (GWB-535B4A)



0.1 ml


Clone ID: EP383Y

Immunogen: A synthetic peptide of human near the C-term of PI3-K p110 alpha was used as an immunogen. Specificity: A synthetic peptide of human near the C-term of PI3-K p110 alpha was used as an immunogen. Dilution: WB: 1:1,000-5,000
ICC: 1:100-250
IP: 1:30. PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; p85 and p10. The p85 subunit localize PI3-K activity to the plasma membrane while the p110 subunit contains the catalytic domain of PI3-K (1-2). Four isoforms of p110 has been found; and the subunit. The alpha isoform, also known as PI3KCA, is a transforming oncogene (3) that was shown to have activating mutations in nine types of cancers such as colon, brain, breast and stomach (4-5). Three hot spot mutations (E542K and E545K; and H1047R) make p110 a potential drug target. 1. Warrington NM, Woerner BM, Daginakatte GC, et al, Spatiotemporal Differences in CXCL12 Expression and Cyclic AMP Underlie the Unique Pattern of Optic Glioma Growth in Neurofibromatosis Type ,Cancer Res 67 (2007) 8588-8595
[Application: WB]
2. Nery FC, Zeng J, Niland BP, et al, TorsinA binds the KASH domain of nesprins and participates in linkage between nuclear envelope and cytoskeleton ,J Cell Sci 121 (2008) 3476-3486
[Application: WB]
3. Kim WY, Chang DJ, Hennessy B, et al, A Novel Derivative of the Natural Agent Deguelin for Cancer Chemoprevention and Therapy ,Cancer Prev Res 1 (2008) 577-587
[Application: WB]
4. Junttila TT, Akita RW, Parsons K, et al, Ligand-Independent HER2/HER3/PI3K Complex Is Disrupted by Trastuzumab and Is Effectively Inhibited by the PI3K Inhibitor GDC-0941 ,Cancer Cell 15 (2009) 429-440
[Application: WB]
5. Jaiswal BS, Janakiraman V, Kljavin NM, et al, PI3-kinase p110 subunit alpha ,Cancer Cell 16 (2009) 463-474
[Application: WB]
6. Levin VA, Panchabhai SC, Shen L, et al, Different Changes in Protein and Phosphoprotein Levels Result from Serum Starvation of High-Grade Glioma and Adenocarcinoma Cell Lines ,J Proteome Res 9 (2010) 179-191
[Application: WB]
1. Otsu et al. (1992) Cell 65, 91-104
2. Hiles et al. (1991) Cell 70, 419-429
3. Chang HW, Aoki M, Fruman D, et al. Science 1997;276:1848
4. Depowski PL, Rosenthal SI, Ross JS. Mod Pathol 2001;14:672
5. Bose S, Crane A, Hibshoosh H, Mansukhani M, Sandweis L, Parsons R, Hum Pathol 2002;33:405
6. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487

Additional Information

Name PI3-kinase p110 subunit alpha Antibody (GWB-535B4A)
Availability Discontinued
Related Product Names PI3-kinase p110 subunit alpha Rabbit Monoclonal Antibody PIK3CA, Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase p110 subunit alpha;PtdIns-3-kPI3-kinase p110 subunit alpha
Swissprot ID P42336
Molecular Weight 110 kDa
Source Rabbit
Application WB, ICC, IP
Clonality Monoclonal
Reactivity Human
Storage Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Datasheets/Manuals Printable datasheet for GWB-535B4A
Intended Use Research Use Only