PI3Ka Protein (GWB-D58E11)

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GWB-D58E11

10-663-45570

1ug
$234.00
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Description

Similarity: Contains 1 PI3K/PI4K domain.

Function: Phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2.

Additional Information

Name PI3Ka Protein (GWB-D58E11)
Related Product Names Phosphoinositide 3-kinase alpha p110a/p85a, PI3Ka, 2.7.1.137, Phosphatidylinositol 3-kinase, 1-phosphatidylinositol 3-kinase, PI3-kinase, PtdIns-3-kinase, Type I phosphatidylinositol kinase, Type III phosphoinositide 3-kinase.; EC 2.7.1.153; PI3-kinase p1
Description of Target Recombinant bovine Phosphoinositide 3-kinase alpha p110a/p85a
Gene Symbol bPI3Ka
Alias Symbols Phosphoinositide 3-kinase alpha p110a/p85a, PI3Ka, 2.7.1.137, Phosphatidylinositol 3-kinase, 1-phosphatidylinositol 3-kinase, PI3-kinase, PtdIns-3-kinase, Type I phosphatidylinositol kinase, Type III phosphoinositide 3-kinase, PI3Ka Bovine, Phos
Swissprot ID P42336
Host Sf9 Insect Cells
NCBI Acc Number NP_006209.2
Product Format PI3Ka solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2 and 50% glycerol. Physical appearance: Sterile filtered liquid formualtion.
Purity Greater than 90.0% as determined by SDS-PAGE.
Format mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2 and 50% glycerol.
Stability PI3Ka although stable at 14 C for 1 week, should be stored desiccated below
-18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Reconstitution and Storage PI3Ka although stable at 14C for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-D58E11
Intended Use Research Use Only
Additional Information Unit Definition: 1 unit is defined as 1 picumole phosphate transferred to PIP2 per minute.
:: Activity: 1,000 U/mg.
:: Product Introduction: PI3Ka plays a specific role in apoptosis in human colon cancer cells. Injection of neutralizing antibodies specific to PI3Ka into adenocarcinoma cells induced apoptosis, a response that was reverted by treating cells with caspase inhibitor. It was also shown that PI3Ka mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3'-phosphorylated phosphoinositides.PI3Ka is a key regulator of the initiation of keratinocyte differentiation. A decrease in PI3Ka activity results in a loss of keratinocyte adhesion to the extracellular membrane and the initiation of early phase differentiation.
Product Description: The PI3Ka catalytic and regulatory subunits are coexpressed in Sf9 insect cells. Phosphoinositide 3-kinase alpha Bovine Recombinant is a glycosilated protein having a molecular weight as follows: p85a chain 83.5 kDa, p110 chain 124.3 kDa.