PI3Kd Protein (GWB-246AAC)

GWB-246AAC

10-663-45573

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Description

Similarity: Contains 1 PI3K/PI4K domain.

Additional Information

Name PI3Kd Protein (GWB-246AAC)
Availability In Stock
Related Product Names Phosphoinositide 3-kinase p110d/p85a, PI3Kd.; EC 2.7.1.153; PI3-kinase p110 subunit delta; PtdIns-3-kinase p110; PI3K; p110delta PI3Kd HumanPIK3CD
Description of Target Recombinant Human Phosphoinositide 3-kinase p110d/p85a
Gene Symbol PI3Kd GST
Alias Symbols Phosphoinositide 3-kinase p110d/p85a, PI3Kd, PI3Kd Human, Phosphoinositide 3-kinase p110d/p85a Human Recombinant
Swissprot ID O00329
Host Sf9 Insect Cells
NCBI Acc Number NP_005017.3
Product Format PI3Kd solution in 25mM Tris-HCl, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol. Physical appearance: Sterile filtered liquid formualtion.
Purity Greater than 95.0% as determined by SDS Page.
Format 0.5mg/ml solution in 25mM Tris, pH 8.0, 50mM NaCl, 0.5mM MgCl2, and 50% glycerol.
Stability PI3Kb although stable at 14 C for 1 week, should be stored desiccated below -18 C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Reconstitution and Storage PI3Kd although stable at 4C for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-246AAC
Intended Use Research Use Only
Additional Information Unit Definition: 1 unit is defined as 1 picumole phosphate transferred to PIP2 per minute.
:: Activity: 3,000 U/mg.
:: Product Description: The class IA PI3K* is expressed in endothelial and in white blood cells. Recently it was shown that the inactivation of PI3K* in bone marrow mast cells (BBMCs) leads to defective stem cell factor-mediated proliferation, adhesion and migration of these cells, and to impaired allergen-IgE induced degranulation and cytokine release. In neutrophils a role for PI3K* in TNFa-induced signaling was demonstrated by a reduction in Aktphosphorylation and PDK1 activity upon treatment with the d-specific inhibitor IC87114. The catalytic subunit carries a GST-Tag which facilitates the protein's application in typical GST pull-down assays and the heterodimer was purified by affinity chromatography. The molecular weight for p110* chain is 120.2 kDa (without Tag) and the p85* chain 83.5 kDa.