PI3KgHis Protein (GWB-EB2083)

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GWB-EB2083

10-663-45572

1ug
$360.00
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Description

Similarity: Contains 1 PI3K/PI4K domain.

Function: 3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.

Additional Information

Name PI3KgHis Protein (GWB-EB2083)
Related Product Names Phosphoinositide 3-kinase p110g, PI3Kg.; EC 2.7.1.153; PI3-kinase p110 subunit gamma; PtdIns-3-kinase subunit p110; PI3Kgamma; PI3K; p120-PI3K PI3KgHis HumanPIK3CG
Description of Target Recombinant Human Phosphoinositide 3-kinase p110g
Gene Symbol PI3Kg His
Alias Symbols Phosphoinositide 3-kinase p110g, PI3Kg, PI3KgHis Human, Phosphoinositide 3-kinase p110g Human Recombinant
Swissprot ID P48736
Host Sf9 Insect Cells
NCBI Acc Number NP_002640.2
Product Format 0.5 mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2, and 50% glycerol. Physical appearance: Sterile Filtered clear solution.
Purity Greater than 95.0% as determined by SDS-PAGE.
Format 0.5 mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2, and 50% glycerol.
Stability PI3Kg although stable at 14 C for 1 week, should be stored desiccated below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Reconstitution and Storage PI3Kg although stable at 14C for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
Datasheets/Manuals Printable datasheet for GWB-EB2083
Intended Use Research Use Only
:: Biological Activity: ~ 4.5 nmol/mg/min using phosphatidylinositol as the substrate.
:: Product Introduction: PI3Kg is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3Kg-/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8a-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3Kg.
Product Description: Recombinant full length PI3Kg (126.3 kDa without Tag) carries an N-terminal His 8 affinity Tag.