Description
Similarity: Contains 1 PI3K/PI4K domain.
Function: 3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
Function: 3-phosphorylates the cellular phosphoinositide PtdIns-4,5-biphosphate (PtdIns(4,5)P2) to produce PtdIns-3, 4,5-triiphosphate (PtdIns(3,4,5)P3). Links G-protein coupled receptor activation to the secondary messenger PtdIns(3,4,5)P3 production.
Additional Information
| Name | PI3KgHis Protein (GWB-EB2083) |
|---|---|
| Availability | In Stock |
| Related Product Names | Phosphoinositide 3-kinase p110g, PI3Kg.; EC 2.7.1.153; PI3-kinase p110 subunit gamma; PtdIns-3-kinase subunit p110; PI3Kgamma; PI3K; p120-PI3K PI3KgHis HumanPIK3CG |
| Description of Target | Recombinant Human Phosphoinositide 3-kinase p110g |
| Gene Symbol | PI3Kg His |
| Alias Symbols | Phosphoinositide 3-kinase p110g, PI3Kg, PI3KgHis Human, Phosphoinositide 3-kinase p110g Human Recombinant |
| Swissprot ID | P48736 |
| Host | Sf9 Insect Cells |
| NCBI Acc Number | NP_002640.2 |
| Product Format | 0.5 mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2, and 50% glycerol. Physical appearance: Sterile Filtered clear solution. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Format | 0.5 mg/ml solution in 25mM HEPES, pH 8.0, 25mM NaCl, 2.5mM MgCl2, and 50% glycerol. |
| Stability | PI3Kg although stable at 14 C for 1 week, should be stored desiccated below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Reconstitution and Storage | PI3Kg although stable at 14C for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Datasheets/Manuals | Printable datasheet for GWB-EB2083 |
| Intended Use | Research Use Only |
| :: | Biological Activity: ~ 4.5 nmol/mg/min using phosphatidylinositol as the substrate. |
| :: | Product Introduction: PI3Kg is highly expressed in cells of hematopoietic origin. It plays an important role in dendritic cell (DC) trafficking and in the activation of specific immunity. PI3Kg-/- mice showed a reduced ability to respond to chemokines, and had a selective defect in the number of skin Langerhans cells and in lymph node CD8a-DCs. In macrophages, the chemokine RANTES/CCL5 activates the small GTPase Rac1 and its downstream target PAK2. This response depends on Gi activation and largely on the subsequent triggering of PI3Kg. Product Description: Recombinant full length PI3Kg (126.3 kDa without Tag) carries an N-terminal His 8 affinity Tag. |