Human EGF R - FcA Chimera (GWB-1C8287)

GWB-1C8287

10-006-220013

25 ug
$315.00

Description

Source: A DNA sequence encoding the signal peptide and extracellular domain of human EGF R (aa 1-525) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modified human 293 cells.

Molecular Mass: Under reducing conditions EGF R - FcB Chimera migrates as a broad band between 85 and 110 kDa on SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified EGF R - FcB Chimera that has a predicted monomeric molecular mass of 83 kDa.

PI: EGF R - FcB Chimera separates into a number of glycoforms with a pI between 6.0 and 8.5 on 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified EGF R - Fc Chimera that has a predicted pI of 7.8. Theoretical Sequence: LEEKKVCQGT SNKLTQLGTF EDHFLSLQRM FNNCEVVLGN LEITYVQRNY DLSFLKTIQE VAGYVLIALN TVERIPLENL QIIRGNMYYE NSYALAVLSN YDANKTGLKE LPMRNLQEIL HGAVRFSNNP ALCNVESIQW RDIVSSDFLS NMSMDFQNHL GSCQKCDPSC PNGSCWGAGE ENCQKLTKII CAQQCSGRCR GKSPSDCCHN QCAAGCTGPR ESDCLVCRKF RDEATCKDTC PPLMLYNPTT YQMDV Activity: The ED50 of EGF R - FcB Chimera is typically 60 -100 ng/ml as measured by its ability to neutralize EGF mediated proliferation of murine NIH3T3 fribroblasts.

% Carbohydrate: purified EGF R - FcB Chimera consists of 5-30% carbohydrate by weight.

Glycosylation: EGF R - FcB Chimera contains N- and O-linked oligosaccharides.

Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. EGF R, also known as Epidermal growth factor receptor, is a member of the ErbB family receptors. EGF R is present in most, if not all, normal human epithelial cells. EGF R main function is as a receptor for EGF, but it also acts as a receptor for other members of the EGF family such as TGF-alpha, amphiregulin, and heparin-binding EGF-like growth factor. When EGF attaches to human EGF R it activates the enzyme tyrosine kinase, triggering reactions that cause the cells to grow and multiply. EGF R is a 479 amino acid glycoprotein that consists of an extracellular receptor domain, a transmembrane region, and an intracellular domain with tyrosine kinase function.

N/A

N/A [1] Callaghan,T., Antczak,M., Flickinger,T., Raines,M., Myers,M. and Kung,H.J.
A complete description of the EGF-receptor exon structure: implication in oncogenic activation and domain evolution

Additional Information

Name Human EGF R - FcA Chimera (GWB-1C8287)
Related Product Names human cell expressed EGF R - FcA Chimera; N/A Human EGF R - FcA ChimeraEGF R
NCBI Acc Number AAB28540.1
Purity >95%, as determined by SDS-PAGE and visualized by Coomassie Brilliant Blue.
GI Number 439763
Format When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
Reactivity Human
Sequence Length 599
Datasheets/Manuals Printable datasheet for GWB-1C8287
Storage Lyophilized products should be stored at 2 to 8C. Following reconstitution short-term storage at 4C is recommended, with longer-term storage in aliquots at -18 to -20C. Repeated freeze thawing is not recommended.
Intended Use Research Use Only