PTPN13 (FAP-1) , Active (GWB-5F79A3)





Application Note: Kinase assay Recombinant human PTPN13 (2169-2485) was expressed in E.coli cells using a N-terminal GST tag.

Function: Regulates negatively FAS-induced apoptosis and NGFR-mediated pro-apoptotic signaling.

Catalytic Activity: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit: Interacts with TRIP6 and TNFRSF6 (Fas receptor) through its second PDZ domain. Interacts with the C-terminal SVP motif of NGFR through its third PDZ domain. Interacts with the LIM domain of PDLIM4 through its second and fourth PDZ domains. Binds PLEKHA1 and PLEKHA2 through its first PDZ domain. Interacts with BRD7.

Subcellular Location: Cytoplasm (By similarity).

Tissue Specificity: Present in most tissues with the exception of the liver and skeletal muscle. Most abundant in lung, kidney and fetal brain.

Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.

Similarity: Contains 1 FERM domain.

Similarity: Contains 5 PDZ (DHR) domains.

Similarity: Contains 1 tyrosine-protein phosphatase domain.

Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large protein that possesses a PTP domain at C-terminus, and multiple noncatalytic domains, which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains, and a leucine zipper motif. This PTP was found to interact with, and dephosphorylate Fas receptor, as well as IkappaBalpha through the PDZ domains, which suggested its role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathway. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [1] Yeh,S.H., Wu,D.C., Tsai,C.Y., Kuo,T.J., Yu,W.C., Chang,Y.S., Chen,C.L., Chang,C.F., Chen,D.S. and Chen,P.J.
Genetic characterization of fas-associated phosphatase-1 as a putative tumor suppressor gene on chromosome 4q21.3 in hepatocellular carcinoma
[2] Ivanov,V.N., Ronai,Z. and Hei,T.K., et al.
Opposite roles of FAP-1 and dynamin in the regulation of Fas (CD95) translocation to the cell surface and susceptibility to Fas ligand-mediated apoptosis
[3] Savaskan,E., Ravid,R., Meier,F., Muller-Spahn,F. and Jockers,R., et al.
Immunohistochemical Localization of Fas-associated phosphatase-1 (FAP-1) in Alzheimer disease hippocampus
[4] Abaan,O.D., Levenson,A., Khan,O., Furth,P.A., Uren,A. and Toretsky,J.A.
PTPL1 is a direct transcriptional target of EWS-FLI1 and modulates Ewing\'s Sarcoma tumorigenesis
[5] Villa,F., Deak,M., Bloomberg,G.B., Alessi,D.R. and van Aalten,D.M., et al.
Crystal structure of the PTPL1/FAP-1 human tyrosine phosphatase mutated in colorectal cancer: evidence for a second phosphotyrosine substrate recognition pocket
[6] Banville D., Ahmad S., Stocco R., Shen S.-H.
A novel protein-tyrosine phosphatase with homology to both the cytoskeletal proteins of the band 4.1 family and junction-associated guanylate kinases.
[7] Maekawa K., Imagawa N., Nagamatsu M., Harada S.
Molecular cloning of a novel protein-tyrosine phosphatase containing a membrane-binding domain and GLGF repeats.
[8] Saras J., Claesson-Welsh L., Heldin C.-H., Gonez L.J.
Cloning and characterization of PTPL1, a protein tyrosine phosphatase with similarities to cytoskeletal-associated proteins.
[9] Wang H.Y.
Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
[10] Sato T., Irie S., Kitada S., Reed J.C.
FAP-1: a protein tyrosine phosphatase that associates with Fas.

Additional Information

Name PTPN13 (FAP-1) , Active (GWB-5F79A3)
Related Product Names EC; Protein-tyrosine phosphatase 1E; PTP-E1; hPTPE1; PTP-BAS; Protein-tyrosine phosphatase PTPL1; Fas-associated protein-tyrosine phosphatase 1; FAP-1 PNP1; PTP1E; PTPL1Tyrosine-protein phosphatase non-receptor type 13PTPN13
Swissprot ID Q12923
NCBI Acc Number NP_006255.1
Molecular Weight 0.5
Source E Coli
GI Number 5453992
Stability 1 year at -20 degree C.
Sequence Length 2466
Datasheets/Manuals Printable datasheet for GWB-5F79A3
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Intended Use Research Use Only