IL 1RA Protein (GWB-4B6009)

GWB-4B6009

10-663-47052

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Description

Sequence: ThesequenceofthefirstsixN - terminalaminoacidswasdeterminedandwasfoundtobe A la - Cys - A rg - Pro - Ser - Gly.

Additional Information

Name IL 1RA Protein (GWB-4B6009)
Related Product Names IL 1RA Mouse, Interleukin-1 Receptor Antagonist Mouse Recombinant, IRAP, IL1F3, IL1RA, IL-1ra3, ICIL-1RA, IL1RN, IL1 inhibitor, IL-1ra, F630041P17Rik Recombinant Mouse Interleukin-1 Receptor AntagonistmIL 1RA
Gene Symbol IL1RA
Gene id 16181
Alias Symbols IRAP, IL1F3, IL1RA, IL-1ra3, ICIL-1RA, IL1RN, IL1 inhibitor, IL-1ra, F630041P17Rik, IL 1RA Mouse, Interleukin-1 Receptor Antagonist Mouse Recombinant
Swissprot ID P25085
Protein Name Interleukin-1 receptor antagonist protein
Description of Target Recombinant Mouse Interleukin-1 Receptor Antagonist
Protein Accession Num NP_001034790.1
Host E. Coli
Peptide Sequence The sequence of the first six N - terminal amino acids was determined and was found to be Ala - Cys - Arg - Pro - Ser - Gly.
Product Format The protein was lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity Greater than 98.0% as determined by (a) Analysis by Gel Filtration. (b) Analysis by SDS-PAGE.
Format The protein was lyophilized from a concentrated (1mg/ml) solution in water containing NaHCO3.
Reconstitution and Storage Lyophilized Interleukin 1ra although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution IL-1ra should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
Storage Lyophilized Interleukin 1ra although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IL-1ra should be stored at 4C between 2-7 days and for future use below -18 C.For long term storage it is recommended
Datasheets/Manuals Printable datasheet for GWB-4B6009
Intended Use Research Use Only
Additional Information Solubility: It is recommended to reconstitute the lyophilized Interleukin-1ra in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
:: Biological Activity: The mouse IL-1Ra is capable of inhibiting IL-1-alpha activity in helper T cell line D10.G4.1.
:: Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 ra as a Reference Standard.
Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.91 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-1 ra as a Reference Standard.
:: Product Introduction: Interleukin-1 ra is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
Product Description: IL1 ra Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17.4kDa. The IL1ra is purified by proprietary chromatographic techniques.