PI3-kinase p85 subunit alpha (C-term), Antibody



0.1 ml


Clone ID: EP380Y

Immunogen: A synthetic peptide corresponding to residues in the C-term of human PI3-K 85 alpha was used as an immunogen. Specificity: A synthetic peptide corresponding to residues in the C-term of human PI3-K 85 alpha was used as an immunogen. Dilution: WB: 1:500-2000
IHC: 1:100-250
ICC: 1:250-500
FC: 1:60
IP: 1:50. PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; the regulatory subunit p85 and the catalytic subunit p110 (1-2). In quiescent cells p85 maintains p110 in a low activity state. Upon growth factor stimulation, p85 directly interacts with phosphotyrosine residues of activated growth factor receptors or adaptor proteins leading to activation of p110. p85 exists in two isoforms: p85 , including two short forms termed p50 and p55/AS53 and p85 including short form p55 PIK/p55 (3). The p85 adaptor protein uses the inter-SH2 (iSH2) region to bind p110 and employs SH3 and SH2 domains, phosphotyrosine residues, and proline-rich motifs to interact with activated tyrosine kinases such as BCR/ABL and NPM/ALK or adaptor proteins including Shc, c-Cbl, Crk-L, and Gab2 (4-5). 1. Otsu et al. (1992) Cell 65, 91-104
2. Hiles et al. (1991) Cell 70, 419-429
3. Inukai et al. (1997) J. Biol. Chem. 272, 7873-7882
4. Klippel, A., J. A. Escobedo, Q. Hu, and L. T. Williams. Mol. Cell. Biol. 13:5560-5566.
5. Jain, S. K., W. Y. Langdon, and L. Varticovski. 1997.Oncogene 14:2217-2228
6. Manufactured under U.S. Patents, No. 5,675,063 and 7,429,487

Additional Information

Name PI3-kinase p85 subunit alpha (C-term), Antibody
Related Product Names PI3-kinase p85 subunit alpha (C-term) Rabbit Monoclonal Antibody PIK3R1, GRB1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase p85 subunit alpha;PtdIns-3-kinase p85-alphaPI3-kinase p85 subunit alpha (C-term)
Swissprot ID p27986
Molecular Weight 85 kDa
Source Rabbit
Applications WB, IHC, ICC, FC, IP
Clonality Monoclonal
Reactivity Human, Rat
Storage Store at -20C. Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. Stable for 12 months from date of receipt.
Datasheets/Manuals Printable datasheet for GWB-4B79C0
Intended Use Research Use Only