Fluorogenic GCN5 Assay Kit (GWB-2FFDA0)




Description: The Histone Acetyltransferase Assay Kit is a homogeneous, fluorogenic assay designed to screen for inhibitors of histone acetyltransferase (HAT) activity or to measure GCN5 HAT activity using cell extracts or purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for 100 uluorescent HAT activity measurements. No time-consuming washing steps are required. In addition, the kit includes purified GCN5 (also known as KAT2A) for use as a positive control. The fluorogenic Histone Acetyltransferase Assay Kit is based on the transfer of an acetyl group from acetyl CoA to a peptide substrate. After incubation with acetyl CoA and the substrate, the GCN5 enzyme generates acetylated H3 peptide and CoASH. The thiol groups of CoASH can be detected with fluorogenic reagent at excitation = 360 nm and emission = 460 nm. Components: GCN5 (KAT2A) enzyme, 10 ?g; Acetyl CoA (2.5 mM), 100 ul; 10X HAT assay buffer, 5 ml; 52010 H3 peptide, 500 ul; Fluorescence Developer, 100 ul; Developer Solution, 10 ul; Stop Solution, 5 ml; 1 black, low binding NUNC black microtiter plate.

Format: assay kit

Scientific Category: Acetyltransferase/Kit

Additional Information

Name Fluorogenic GCN5 Assay Kit (GWB-2FFDA0)
Related Product Names HAT, histone acetyltransferase, GCN5, KAT
Format Assay Kit
Datasheets/Manuals Printable datasheet for GWB-2FFDA0
Storage Mixed Storage
Intended Use Research Use Only