Lysyl Oxidase-like 4 (LOXL4) Human Recombinant Protein (GWB-931D01)




Description: Human LOXL4 (GenBank Accession No. NM_032211), amino-acids 500-end, with C-terminal Flag-tag, MW = 31 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Activity: Enzyme activity is assayed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL-4 (0.25-1 ng/μl) is incubated with 3 mM 1,5-diaminopentane, 0.4 mM AmplexRed and 0.2 unit/ul peroxidase in 100 ul of the assay buffer (1.2M Urea, 50 mM borate pH=8.8) for 15 min at 30C. Fluorescence intensity is measured every 7 minute at 530ex/590em. Fomulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. Stability: >6 months at -80C. References: 1. Gorogh T. et al., Int. J. Oncol. 33 (5), 1091-1098 (2008). 2. Kim D.J. et al., Biochem. Biophys. Res. Commun. 373 (4), 521-527 (2008).

Host: baculovirus

Tag: C-terminal Flag-tag

GI Num: NM_032211

MW: 31 kDa

Format: aqueous buffer solution

Scientific Category: Lysyl Oxidase

Species: human

Additional Information

Name Lysyl Oxidase-like 4 (LOXL4) Human Recombinant Protein (GWB-931D01)
Related Product Names Lysyl Oxidase-like 4 (LOXL4) Human Recombinant Protein
Molecular Weight 31 kDa
Source baculovirus
GI Number NM_032211
Format Aqueous Buffer Solution
Reactivity Human
Storage -80C
Datasheets/Manuals Printable datasheet for GWB-931D01
Intended Use Research Use Only