PDE Assay Kit (GWB-58EAA2)


96 reactions


Description: The PDE Assay Kit is designed for identification of PDE inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDEs to the binding agent. PDEs plays an important role in dynamic regulation of cAMP and cGMP signaling. The PDE inhibitor screening assay kit comes in a convenient 96-well format, with fluorescently labeled substrate (cAMP and cGMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE reactions. First, the fluorescently labeled cAMP or cGMP is incubated with a sample containing PDEs for 1h. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader. COMPONENTS: PDE assay buffer: 6 ml FAM-Cyclic-3?,5'-AMP: 50 ul @ 20 uM FAM-Cyclic-3?,5?-GMP: 50 ul @ 20 uM Binding agent: 100 ul Binding agent diluent: 10 ul 1 black plate 20 ng PDE2A, a positive control for cAMP reaction 20 ng PDE5A, a positive control for cGMP reaction APPLICATIONS: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. STORAGE: -20C or -70C. REFERENCES: 1. Maurice DH. Front Biosci. 2005; 10:1221-8.

Format: assay kit

Scientific Category: PDE

Additional Information

Name PDE Assay Kit (GWB-58EAA2)
Related Product Names PDE, phosphodiesterase, screening, inhibitors, universal
Format Assay Kit
Datasheets/Manuals Printable datasheet for GWB-58EAA2
Storage Mixed storage
Intended Use Research Use Only