PDE10A Assay Kit (GWB-2583E5)


96 reactions


Description: The PDE10A Assay Kit is designed for identification of PDE10A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE10A to the binding agent. PDE10A is a dual substrate PDE highly expressed in striatal medium spiny neurons. PDE10A inhibitors have the potential to improve the cognitive symptoms of schizophrenia. The PDE10A inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE10A enzyme, fluorescently labeled PDE10A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE10A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE10A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE10A for 1h. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader. COMPONENTS: PDE assay buffer: 6 ml FAM-Cyclic-3?,5'-AMP: 50 ul @ 20 uM Binding agent: 100 ul Binding agent diluent: 10 ul 1 black plate 100 ng PDE10A for 100 reactions APPLICATIONS: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. STORAGE: -20C or -70C. REFERENCES: 1. Threlfell S, Sammut S, Menniti FS, Schmidt CJ, West AR. J Pharmacol Exp Ther 2009; 328(3):785-95. 2. Menniti FS, Chappie TA, Humphrey JM, Schmidt CJ. Curr Opin Investig Drugs 2007; 8(1):54-9.

Format: assay kit

Scientific Category: PDE

Additional Information

Name PDE10A Assay Kit (GWB-2583E5)
Related Product Names PDE, phosphodiesterase, screening, inhibitors, PDE10A
Format Assay Kit
Datasheets/Manuals Printable datasheet for GWB-2583E5
Storage Mixed storage
Intended Use Research Use Only