PDE4B2 Assay Kit (GWB-A8C9F0)




Description: The PDE4B2 Assay Kit is designed for identification of inhibitors of PDE4B2 using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE4B2 to the binding agent. The PDE4B2 inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE4B2 enzyme, fluorescently labeled PDE4B2 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4B2 Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4B2 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4B2 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader. COMPONENTS: PDE4B2 recombinant enzyme, 1 ug; FAM-Cyclic-3?,5'-AMP: 20 uM, 50 ul; PDE assay buffer, 20 ul; Binding Agent 100 ul; Binding Agent Diluent (cAMP) 10 ul; Black, low binding microtiter plate. APPLICATIONS: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. STORAGE: -20C or -70C. REFERENCES: 1. Cullen MD, et al. Bioorg Med Chem Lett. 2008; 18(4):1530-3. 2. Mehats C, et al. BMC Pregnancy Childbirth. 2007; 7 Suppl 1:S12.

Format: assay kit

Scientific Category: PDE

Additional Information

Name PDE4B2 Assay Kit (GWB-A8C9F0)
Related Product Names PDE, phosphodiesterase, screening, inhibitors, PDE4B
Format Assay Kit
Storage Mixed storage
Datasheets/Manuals Printable datasheet for GWB-A8C9F0
Intended Use Research Use Only