Mitochondrial Antigen, M2 Protein (GWB-T00061)

Description Mitochondrial Antigen Mitochondrial Antigen M2 Mitochondrial antigen M2 is a cluster of four major mitochondrial innermembrane proteins of approximately 74, 52, 45 and 39 kDa. Contains the E2 subunit of branched chain 2-oxo acid dehydrogenase, the E2 subunit of the 2-oxo dehydrogenase complex, protein X and the E1a and E1b subunits of the pyruvate dehydrogenase complex. Other antigens (Sm, RNP, SSA, Jo-1 and SSB) are not detected with ELISA assays.

Source Mixed Bovine Spleen and Thymus

Format Affinity Purified, Liquid

Purification Purified using immobilized human anti-Mitochondrial antibodies

Concentration Lot specific Units/ml One unit of activity is the amount of antigen that will generate an absorbance of 1.2 when assayed using a standard procedure for enzyme immunoassay. Activity will vary with assay conditions.

Buffer 0.01M Tris containing 0.53M Sodium chloride

Preservative 0.02% Sodium azide

Application Suitable for use in ELISA. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Storage Store at -70C + 10C. Aliquot to avoid multiple freeze/thaw cycles.

Inactivation Not applicable

Warning This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1 - 1.0 %. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.

References The references listed below are for research purposes only. 1. Baum, H., et al., (1985), Mol. Aspects Med., 8:201-234 2. "Molecular basis of mitochondrial autoreactivity in primary biliary cirrhosis", Immunology Today, Vol. 10 (1989) 3. American College of Rheumatology, 55th Annual Scientific Meeting, Boston MA, Poster Presentation (1991)