Purified Plasma Kallikrein

GWB-1848B4
11-511-248753
0.05 mg
$153
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Description

Description Purified Plasma Kallikrein, Purified Human Plasma Kallikrein
Inactivation Not applicable.
Buffer Lyophilized from 20mM Tris-HCl, pH 7.8 containing 100mM Sodium chloride.
Applications Notes Specific methodologies have not been tested using this product.
Warnings All human source materials have tested negative for HIV1, HIV2, HBc, HCV antibodies and HBsAg. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.
Concentration Concentration is lot specific.
Preservative None Purified Plasma Kallikrein. Purified Human Plasma Kallikrein.
Function The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Catalytic Activity Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.
Subunit The zymogen is activated by factor XIIa, which cleaves the molecule into a light chain, which contains the active site, and a heavy chain, which associates with HMW kininogen. These chains are linked by one or more disulfide bonds.
Subcellular Location Secreted.
Disease Defects in KLKB1 are the cause of Fletcher factor deficiency [MIM:229000]; a blood coagulation defect.
Similarity Belongs to the peptidase S1 family. Plasma kallikrein subfamily [view classification].
Similarity Contains 4 apple domains.
Similarity Contains 1 peptidase S1 domain [view classification].

Additional Information

Name Purified Plasma Kallikrein
Related Product Names Purified Plasma Kallikrein; EC 3.4.21.34; Plasma prekallikrein; Kininogenin; Fletcher factor KLK3Purified Plasma KallikreinKLKB1
Intended Use Research Use Only
NCBI Acc Number NP_000883.2
Purity >95% pure (SDS-PAGE)
Format Purified, Lyophilized. Reconstitute with 109.2ul distilled water.
Storage Store at <= - 70°C . (reconstituted form)
Molecular Weight 71370
Swiss Prot Number P03952
Concentration 0.435mg/mL (prior to lyophilization) (OD280nm, E1%=10.6) > 15 units per mg. One unit is defined as the amount of enzyme that will hydrolyze 1μmole of D-Pro-Phe-Arg-pNA (Chromogenix S-2302) per minute at 25°C, pH 7.8.