Clusterin Protein (GWB-246D15)

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GWB-246D15

10-663-45754

2ug
$59.00
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Description

Description: The Clusterin Human contains a total of 438 amino acids and a calculated molecular mass of 51.27kDa (calculated). The AA sequence (AA 1-427) is identical to Swiss-ProtP10909 (AA 23-449, secreted Human Clusterin). C-terminal Flag-tag 11 extra AA (underlined). The Clusterin Human contains a total 454 amino acids and a calculated molecular mass of 53058Da. The AA sequence (AA 1-427) is identical to Swiss-Prot-P10909 (AA 23-449, secreted Human Clusterin). C-terminal His tag 27AA (bold).

Solubility: Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyphilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

Similarity: Belongs to the clusterin family.

Function: Not yet clear. It is known to be expressed in a variety of tissues and it seems to be able to bind to cells, membranes and hydrophobic proteins. It has been associated with programmed cell death (apoptosis).

Additional Information

Name Clusterin Protein (GWB-246D15)
Related Product Names CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903.; Complement-associated protein SP-40,40; Complement cytolysis inhibitor; CLI; NA1/NA2; Apolipoprotein J; Apo-J; Testosterone-repressed prostate message 2; TRPM-2; Ku70-binding protein 1 APOJ; CLI; KUB1
Description of Target Recombinant Human Apoliprotein-J
Gene Symbol Clusterin
Gene id 1191
Alias Symbols CLI, AAG4, KUB1, SGP2, SGP-2, SP-40, TRPM2, MGC24903, Clusterin, Apolipoprotein J, Apo-J, Clusterin Human, Clusterin Human Recombinant
Swissprot ID P10909
Protein Name Clusterin
Protein Accession Num NP_001164609.1
Host 293 Cell Line (Human Embryonic Kidney)
Peptide Sequence DQTVSDNELQ EMSNQGSKYV NKEIQNAVNG VKQIKTLIEK TNEERKTLLS NLEEAKKKKE DALNETRESE TKLKELPGVC NETMMALWEE CKPCLKQTCM KFYARVCRSGS GLVGRQLEE FLNQSSPFYF WMNGDRIDSL LENDRQQTHM LDVMQDHFSRA SSIIDELFQ DRFFTREPQD TYHYLPFSLP HRRPHFFFPK SRIVRSLMPF SPYEPLNFHA MFQPFLEMIH EAQQAMDIHF HSPAFQHPPT EFIREGDDDR TVCREIRHNS TGCLRMKDQC DKCREILSVD CSTNNPSQAKLRRELDESLQ VAERLTRKYN ELLKSYQWKM LNTSSLLEQL NEQFNWVSRL ANLTQGEDQYYLRVTTVASH TSDSDVPSGV TEVVVKLFDS DPITVTVPVE VSRKNPKFME TVAEKALQEY RKKHREEGSL GSGWSHPQFE KTGHHHHHHH HGGQ.
NCBI Acc Number NP_001822.2
Product Format Filtered (0.4 micron) and lyophilized PBS, pH 7.5.
Purity Greater than 95% (SDS PAGE analyzed).
Purification: Ni-NTA chromatography.
Format Filtered (0.4 micron) and lyophilized PBS, pH 7.5.
Stability Store lyophilized protein at -20 C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 C for a limited period of time; it does not show any change after two weeks at 4 C.
The lyo
Reconstitution and Storage Store lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable until the expiry date when stored at -20C.
Datasheets/Manuals Printable datasheet for GWB-246D15
Lead Time Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Intended Use Research Use Only
Additional Information Solubility: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.
:: Product Introduction: Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.The precursor polypeptide chain is cleaved proteolytically to remove the 22-mer secretory signal peptide and subsequently between residues 227/228 to generate the a and b chains. These are assembled in anti-parallel to give a heterodimeric molecule in which the cysteine-rich centers are linked by five disulfide bridges and are flanked by two predicted coiled-coil a-helices and three predicted amphipathic a-helices.Across a broad range of species clusterin shows a high degree of sequence homology ranging from 70% to 80%. It is nearly ubiquitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen.It is able to bind and form complexes with numerous partners such as immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin and others. Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition.A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced insults caused by degraded and misfolded protein precipitates.Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others.
Product Description: The Clusterin Human contains a total 454 amino acids and a calculated molecular mass of 53058Da. The AA sequence (AA 1-427) is identical to Swiss-Prot-P10909 (AA 23-449, secreted Human Clusterin). C-terminal His tag 27AA (bold).