Regulator of G-protein signaling 2 (GWB-1DE0F0)





Fusion: T7 tag at N-terminus. Protocols from Publications
Citation: 1: Nguyen CH, Ming H, Zhao P, Hugendubler L, Gros R, Kimball SR, Chidiac P. Translational control by RGS2. J Cell Biol. 2009 Sep 7;186(5):755-65. PubMed PMID: 19736320; PubMed Central PMCID: PMC2742185.

Experiment Name: Coimmunoprecipitation of endogenous RGS2 with endogenous eIF2B?.

Experiment Background: RGS2 was capable of interfering with the eIF2-eIF2B GTPase cycle, which is a requisite step for the initiation of mRNA translation.

Experiment Steps: 1. Treat UMR-106 osteoblastlike osteosarcoma cells for for 3 h with 100 ?M forskolin to induce RGS2 expression.
2. Incubate forskolin-treated cell lysates (1 mg total protein) with protein A/G agarose beads either without (lanes 1 and 3) or with (lanes 2 and 4) anti eIF2B? antibody.
3. Wash Protein A/G agarose beads extensively and remove the bound protein by heating
4. Perfrom SDSPAGE.
5. Transfer SDS-PAGE gel to PVDF membrane.
6. Probe membrane with anti-RGS2 antibody.
7. Reprobe using the same eIF2B? antibody.
8. visualize blots by chemiluminescence.
Function: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. May play a role in leukemogenesis.

Tissue Specificity: Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).

Ptm: Phosphorylated by protein kinase C.

Similarity: Contains 1 RGS domain. [1] Riddle,E.L., Rana,B.K., Murthy,K.K., Rao,F., Eskin,E., O\'Connor,D.T. and Insel,P.A.
Polymorphisms and haplotypes of the regulator of G protein signaling-2 gene in normotensives and hypertensives
[2] Hague,C., Bernstein,L.S., Ramineni,S., Chen,Z., Minneman,K.P. and Hepler,J.R.
Selective inhibition of alpha1A-adrenergic receptor signaling by RGS2 association with the receptor third intracellular loop
[3] Beckers,J., Herrmann,F., Rieger,S., Drobyshev,A.L., Horsch,M., Hrabe de Angelis,M. and Seliger,B.
Identification and validation of novel ERBB2 (HER2, NEU) targets including genes involved in angiogenesis
[4] Wang,X., Zeng,W., Soyombo,A.A., Tang,W., Ross,E.M., Barnes,A.P., Milgram,S.L., Penninger,J.M., Allen,P.B., Greengard,P. and, et al.
Spinophilin regulates Ca2+ signalling by binding the N-terminal domain of RGS2 and the third intracellular loop of G-protein-coupled receptors
[5] Schwable,J., Choudhary,C., Thiede,C., Tickenbrock,L., Sargin,B., Steur,C., Rehage,M., Rudat,A., Brandts,C., Berdel,W.E.,, et al.
RGS2 is an important target gene of Flt3-ITD mutations in AML and functions in myeloid differentiation and leukemic transformation
[6] , Siderovski D.P., Heximer S.P., Forsdyke D.R.
A human gene encoding a putative basic helix-loop-helix phosphoprotein whose mRNA increases rapidly in cycloheximide-treated blood mononuclear cells.
[7] , Puhl H.L. III, Ikeda S.R., Aronstam R.S.
cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (
[8] , Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., et al.
Cloning of human full-length CDSs in BD Creator(TM) system donor vector.
[9] , Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., et al.
The DNA sequence and biological annotation of human chromosome 1.
[10] , Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., et al.
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.

Additional Information

Name Regulator of G-protein signaling 2 (GWB-1DE0F0)
Related Product Names RGS2; G0/G1 switch regulatory protein 8 G0S8Regulator of G-protein signaling 2RGS2
Swissprot ID P41220
NCBI Acc Number NP_002914.1
Molecular Weight 24382
Purity 95%
Source E Coli
Application MS, SDS
GI Number 4506517
Format 10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3.
Stability 6-12 months at -70 degree C.
Reactivity human
Sequence Length 211
Chrom Location 1q31
Datasheets/Manuals Printable datasheet for GWB-1DE0F0
Storage -70 degree C. Avoid frequent freeze and thaw.
Intended Use Research Use Only