PKC?, Active (GWB-1B87A3)





Description: Recombinant full-length human PKCmu was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Formulation: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Specific Activity: 640 nmol/min/mg

Application Note: Kinase assay Recombinant full-length human PKCmu was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag.

Function: This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.

Catalytic Activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme Regulation: Activated by diacylglycerol and phorbol esters.

Subunit: Interacts via its N-terminal region with CENTA1.

Ptm: Autophosphorylated (By similarity).

Similarity: Belongs to the Ser/Thr protein kinase family. PKC subfamily.

Similarity: Contains 1 PH domain.

Similarity: Contains 2 phorbol-ester/DAG-type zinc fingers.

Similarity: Contains 1 protein kinase domain.

Summary: Members of the protein kinase C (PKC) family function in many extracellular receptor-mediated signal transduction pathways. See PRKCA (MIM 176960) for further background information. The PRKCM gene encodes a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.[supplied by OMIM].

[1] Storz,P., Doppler,H. and Toker,A., et al.
Protein kinase D mediates mitochondrion-to-nucleus signaling and detoxification from mitochondrial reactive oxygen species
[2] Wong,C. and Jin,Z.G., et al.
Protein kinase C-dependent protein kinase D activation modulates ERK signal pathway and endothelial cell proliferation by vascular endothelial growth factor
[3] Zhang,W., Zheng,S., Storz,P. and Min,W., et al.
Protein kinase D specifically mediates apoptosis signal-regulating kinase 1-JNK signaling induced by H2O2 but not tumor necrosis factor
[4] Doppler,H., Storz,P., Li,J., Comb,M.J. and Toker,A., et al.
A phosphorylation state-specific antibody recognizes Hsp27, a novel substrate of protein kinase D
[5] Vega,R.B., Harrison,B.C., Meadows,E., Roberts,C.R., Papst,P.J., Olson,E.N. and McKinsey,T.A.
Protein kinases C and D mediate agonist-dependent cardiac hypertrophy through nuclear export of histone deacetylase 5
[6] Johannes F.-J., Prestle J., Eis S., Oberhagemann P., Pfizenmaier K.
PKCmu is a novel, atypical member of the protein kinase C family.
[7] Zemlickova E., Dubois T., Kerai P., Clokie S., Cronshaw A.D., Wakefield R.I.D., Johannes F.-J., Aitken A.
Centaurin-alpha(1) associates with and is phosphorylated by isoforms of protein kinase C.

Additional Information

Name PKC?, Active (GWB-1B87A3)
Related Product Names EC; nPKC-D1; Protein kinase D; Protein kinase C mu type; nPKC-mu PKD; PRKCMSerine/threonine-protein kinase D1PRKD1
Swissprot ID Q15139
NCBI Acc Number NP_002733.1
Molecular Weight 0.5
Purity >90%
Source Insect Cells
GI Number 4506075
Stability 1 year at -70C from date of shipment
Sequence Length 912
Datasheets/Manuals Printable datasheet for GWB-1B87A3
Storage Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
Intended Use Research Use Only