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SRC (His-tag), Active Human Recombinant Protein


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Description: Human SRC (GenBank Accession No. NM_005417), full length with N-terminal HIS tag, MW = 60.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 205 pmol/min/µg Assay condition: 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 0.01% Brij-35, 200 µM ATP, 2 µM Tyr2 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Stability:>6 months at -80°C. References: 1. Finlay, D. and Vuori, K. Cancer Res. 67 (24), 11704-11711 (2007). 2 Vielreicher, M. et al. J. Biol. Chem. 282 (46), 33623-33631 (2007).

Host: baculovirus

Tag: N-terminal His-tag

GI Num: NM_005417

MW: 60.5 kDa

Format: aqueous buffer solution

Formula: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, and 50% glycerol

Scientific Category: Kinase/Cytoplasmic Tyr

Species: human

Additional Information

Name SRC (His-tag), Active Human Recombinant Protein
Related Product Names SRC (His-tag), Active Human Recombinant Protein
Molecular Weight 60.5 kDa
Datasheets / Downloads GWB-1606DC Datasheet
Source baculovirus
GI Number NM_005417
Format Aqueous Buffer Solution
Reactivity Human
Storage -80°C
Intended Use Research Use Only