TLR7 Over-expression Lysate Product (GWB-100C5E)



0.1 mg
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Additional Information

Name TLR7 Over-expression Lysate Product (GWB-100C5E)
Related Product Names TLR7,TLR7 Over-expression Lysate ProductTLR7
Kit Component -1 vial whole cell lysate (100 ug in 100 ul RIPA buffer)
-1 vial Lysate control (100 ug in 100 ul RIPA buffer)
-1 vial 2X SDS Sample Buffer (250 ul)
NCBI Acc Number NM_016562.3
Product Format Liquid. 25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1mM EDTA, 1X Protein inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4
Molecular Weight 120.7 kDa
Application WB
Preparation SDS-PAGE: Mix equal volume of overexpression lysates with 2xSDS sample buffer. Boil the sample fro 10 mins before loading (In case of membrane proteins, incubate the mixture at room temperature for 30 mins before loading).
Reconstitution and Storage The lysate is shipped with dry ice. Upon receiving, store the sample at -20°C. Avoid repeated freeze/thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20°C for long-term storage. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Datasheets/Manuals Printable datasheet for GWB-100C5E
Intended Use Research Use Only