Description
Short Description: In this assay the Alpha 2-Macroglobulin present in serum sample reacts with the anti-A2M. antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound serum proteins by washing, anti-A2M. antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum A2M. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 2-Macroglobulin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 2-Macroglobulin in the test sample. The quantity of Alpha 2-Macroglobulin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for serum dilution.
Additional Information
| Name | Rat Alpha 2-Macroglobulin ELISA Kit (GWB-98EFFB) | ||||||||||||||||
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| Related Product Names | ALPHA 2M. CPAMD5; Rat Alpha 2-Macroglobulin ELISA Kit; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 CPAMD5Rat Alpha 2-Macroglobulin A2M | ||||||||||||||||
| Kit Range | 6.25 ng/ml - 400 ng/ml | ||||||||||||||||
| Sensitivity | 2.330 ng/ml | ||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
| Alias Symbols | A2M, Alpha-2-macroglobulin, CPAMD5 | ||||||||||||||||
| Swissprot ID | Q61838 | ||||||||||||||||
| Protein Name | Pregnancy zone protein | ||||||||||||||||
| Description of Target | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, | ||||||||||||||||
| Kit Principle | Genway Biotech Alpha-2 Macroglobulin ELISA Kit (Rat) (GWB-98EFFB) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Pregnancy zone protein has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Pregnancy zone protein - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pregnancy zone protein captured in the well. | ||||||||||||||||
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| Assay Info | Assay Methodology: Quantitative Sandwich ELISA | ||||||||||||||||
| Homology | Rat | ||||||||||||||||
| NCBI Acc Number | NP_000005.2 | ||||||||||||||||
| Standard Range | Refer To Manual | ||||||||||||||||
| Product Format | In Solution | ||||||||||||||||
| Specimen/Volumes | Refer To Manual | ||||||||||||||||
| Application | ELISA | ||||||||||||||||
| Reconstitution and Storage | 1. DILUENT The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C. 2. WASH SOLUTION The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C. 3. ENZYME-ANTIBODY CONJUGATE Undiluted horseradish peroxidase anti-A2M conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours. 4. CHROMOGEN-SUBSTRATE SOLUTION The Substrate Solution should be stored at 4-8C and is stable until the expiration date. 5. STOP SOLUTION The Stop Solution should be stored at 4-8C and is stable until the expiration date. 6. ANTI-RAT ALPHA 2-MACROGLOBULIN ELISA MICRO PLATE Anti-Rat A2M coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack. 7. RAT ALPHA 2-MACROGLOBULIN CALIBRATOR The lyophilized Rat Alpha 2-Macroglobulin calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours. |
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| Reactivity | Rat | ||||||||||||||||
| Kit Size | 96 well | ||||||||||||||||
| Kit Duration | 80 Minutes | ||||||||||||||||
| Datasheets/Manuals | Printable datasheet for GWB-98EFFB | ||||||||||||||||
| Storage | Refer to kit instructions for component storage | ||||||||||||||||
| Species Reactivity | Rat | ||||||||||||||||
| Lead Time | Domestic: within 1 week delivery | International: 1 week | ||||||||||||||||
| Intended Use | Research Use Only | ||||||||||||||||
| Additional Information | Intended Use: The Alpha 2-Macroglobulin test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Alpha 2-Macroglobulin in biological samples of rats. | ||||||||||||||||
| :: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Rat Alpha 2-Macroglobulin antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-RAT ALPHA 2-MACROGLOBULIN ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat A2M. 7. RAT ALPHA 2-MACROGLOBULIN CALIBRATOR One vial containing a lyophilized Rat Alpha 2-Macroglobulin calibrator. |