Rat Antithrombin III ELISA Kit

GWB-790BE4

40-374-130023

96 Wells
$539.00
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Description

Short Description: In this assay the ANTITHROMBIN III present in the sample reacts with the anti-ANTITHROMBIN III antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-AT3 antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound serum AT3. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of AT3 in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of AT3 in the test sample. The quantity of AT3 in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Additional Information

Name Rat Antithrombin III ELISA Kit
Availability In Stock
Related Product Names ATIII. AT3; Rat Antithrombin III ELISA Kit; ATIII AT3Rat Antithrombin IIISERPINC1
CB Species Rat
Kit Range 12.5 ng/ml - 400 ng/ml
Sensitivity 1.399 ng/ml
Sample Type Serum, plasma and other biological fluids
Swissprot ID P01008
Protein Name Thrombin III
Kit Principle Genway Biotech Thrombin III ELISA Kit (Rat) (GWB-790BE4) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Thrombin III has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Thrombin III - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Thrombin III captured in the well.
Kit Component
ComponentAmount
Anti-Rat anti Thrombin III Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat anti Thrombin III Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Rat
NCBI Acc Number NP_000479.1
Standard Range Refer To Manual
Product Format In Solution
Specimen/Volumes Refer To Manual
Application ELISA
Reconstitution and Storage 1. DILUENT
The 5X Diluent Concentrate is stable until the expira-tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expira¬tion date. The 1X working solution is stable for at least one week from the date of preparation. Both solutions can be stored at room temperature (16 25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-AT3 conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 8 hours.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-RAT ANTITHROMBIN III ELISA MICRO PLATE
Anti-Rat AT3 coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. RAT ANTITHROMBIN III CALIBRATOR
The lyophilized Rat Antithrombin III calibrator should be stored at 4C or frozen until reconstituted. The reconstituted calibrator should be aliquoted out and stored frozen (Avoid multiple freeze-thaw cycles). The working standard solutions should be prepared immediately prior to use and are stable for up to 8 hours.
Reactivity Rat
Kit Size 96 well
Kit Duration 100 Minutes
Storage Refer to kit instructions for component storage
Datasheets/Manuals Printable datasheet for GWB-790BE4
Species Reactivity Rat
Intended Use Research Use Only
Additional Information Intended Use: The Antithrombin III test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Antithrombin III in biological samples of rats.
:: Reagents:
1. DILUENT CONCENTRATE (Running Buffer)
One bottle containing 50 ml of a 5X concentrated diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 ul of affinity purified anti-Rat Antithrombin III antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT ANTITHROMBIN III ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat AT3.
7. RAT ANTITHROMBIN III CALIBRATOR
One vial containing a lyophilized Rat Antithrombin III calibrator.