Rat L-FABP ELISA (GWB-374Z43) (Aviva Cat. No. OKIA00165)

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GWB-374Z43

Aviva Cat. No.: OKIA00165

Additional Information

Name Rat L-FABP ELISA (GWB-374Z43)
Related Product Names Rat Liver Fatty Acid Binding Protein ELISA, Fatty acid-binding protein, liver, Fabp1, Fabpl, SCP, Z-protein, p14, 14 kDa selenium-binding protein, Fatty acid-binding protein 1, Liver-type fatty acid-binding protein
Kit Range 3.125 ng/ml - 200 ng/ml
Sensitivity 1.278 ng/ml
Sample Type Serum, plasma and other biological fluids
Gene Symbol FABP1
Gene Fullname fatty acid binding protein 1
Gene id 24360
Alias Symbols Fatty acid-binding protein, liver, FABP1
Swissprot ID P02692
Protein Name Fatty acid-binding protein, liver
Description of Target Plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes. Binds cholesterol. Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transpor
Kit Principle Genway Biotech L-FABP ELISA Kit (Rat) (GWB-374Z43) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Fatty acid-binding protein, liver has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Fatty acid-binding protein, liver - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Fatty acid-binding protein, liver captured in the well.
Kit Component
ComponentAmount
Anti-Rat L-FABP Microplate96 Wells (12 x 8 Well Strips)
20X Diluent Concentrate50 mL
20X Wash Solution50 mL
100X Enzyme-Antibody Conjugate150 uL
Chromogen-Substrate Solution12 mL
Stop Solution12 mL
Rat L-FABP Calibrator1
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Homology Rat
Standard Range Refer To Manual
Specimen/Volumes Refer To Manual
Application ELISA
Format ELISA Kit
Reconstitution and Storage 1. DILUENT
The 1X Diluent is stable until the expiration date. The solution should be stored at 4-8C.
2. WASH SOLUTION
The 20X Wash Solution Concentrate is stable until the expiration date. The 1X working solution is stable for at least one week from the date of preparation.
Both solutions can be stored at room temperature (16-25C) or at 4-8C.
3. ENZYME-ANTIBODY CONJUGATE
Undiluted horseradish peroxidase anti-FABP conjugate should be stored at 4-8C and diluted immediately prior to use. The working conjugate solution is stable for up to 1 hour when stored in the dark.
4. CHROMOGEN-SUBSTRATE SOLUTION
The Substrate Solution should be stored at 4-8C and is stable until the expiration date.
5. STOP SOLUTION
The Stop Solution should be stored at 4-8C and is stable until the expiration date.
6. ANTI-RAT FABP ELISA MICRO PLATE
Anti-Rat FABP coated wells are stable until the expiration date, and should be stored at 4-8C in sealed foil pouch with desiccant pack.
7. RAT FABP CALIBRATOR
Long Term Storage: Upon receipt, aliquot the calibrator and store them frozen. They will be stable until expiration date. Short Term Storage: the calibrator is stable for up to 14 days at 4C. The working standard solutions should be prepared immediately prior to use for each run.
INDICATIONS OF INSTABILITY
If the test is performing correctly, the results observed with the standard solutions should be within 20 % of the expected values
Reactivity Rat
Kit Size 96 well
Kit Duration 80 Minutes
Datasheets/Manuals Printable datasheet for GWB-374Z43
Storage Refer to kit instructions for component storage
Species Reactivity Rat
Lead Time Domestic: within 1 week delivery | International: 1 week
Intended Use Research Use Only
Additional Information INTENDED USE
The Fatty Acid Binding Protein (FABP) test kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring FABP in biological samples of Rat.
:: REAGENTS
1. DILUENT (Running Buffer)
One bottle containing 60 ml of a 1X diluent running buffer.
2. WASH SOLUTION CONCENTRATE
One bottle containing 50 ml of a 20X concentrated wash solution.
3. ENZYME-ANTIBODY CONJUGATE 100X
One vial containing 150 L of affinity purified anti-Rat FABP antibody conjugated with horseradish peroxidase in a stabilizing buffer.
4. CHROMOGEN-SUBSTRATE SOLUTION
One vial containing 12 mL of 3,3’,5,5’- tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3.
5. STOP SOLUTION
One vial containing 12 ml 0.3 M sulfuric acid.
WARNING: Avoid contact with skin.
6. ANTI-RAT FABP ELISA MICRO PLATE
Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Rat FABP.
7. RAT FABP CALIBRATOR
One vial containing Rat FABP Calibrator.