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STREPTAVIDIN:RPE Reagent

GWB-Q00336

1 ml
$250.00
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Additional Information

Name STREPTAVIDIN:RPE Reagent
Related Product Names STREPTAVIDIN:RPE Reagent, Flow Cytometry, STREPTAVIDIN:RPE
Description of Target STREPTAVIDIN:RPE
Product Format Streptavidin conjugated to R-Phycoerythrin - lyophilised
Applications Flow Cytometry
Application Info Flow Cytometry:    Neat
Format RPE
Stability 12 months from date of reconstitution.
Reconstitution and Storage Reconstitution: Reconstitute with 1ml distilled water
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should th
Datasheets/Manuals Printable datasheet for GWB-Q00336
Intended Use Research Use Only
Key Reference 1. Van der Nieuwenhof, I. et al. (2001) Differential galactosylation of neuronal and haematopoietic signal regulatory protein-alpha determines its cellular binding - specificity. J. Cell. Sci. 114 (7): 1321 - 1329
2. Tasker, L. et al. (1997) Immature B cells from neonatal mice show a selective inability to up-regulate MHC class II expression in response to antigen receptor ligation. Int. Immunol. 9: 475 - 484.
Additional Information Buffer solution: Phosphate buffered saline pH7.2
:: Label Protein Molar Ratio: Pure Streptavidin is reacted with Succinimidyl 4- (N-maleimido-methyl) cyclohexane - 1- carboxylate (SMCC). R. Phycoerythrin is reacted with N-succinimidyl 3-(2 - pyridyldithio) propionate (SPDP) and activated by reduction with dithiothreitol. The two substituted proteins are reacted together to give covalent conjugates, which are selected by medium pressure liquid chromatography on gel filtration columns of AcA34 ultrogel.
This product conjugates show negligible non-specific binding to non-biotinylated macromolecules, and therefore gives very low backgrounds.
Preservative: 0.09% - Sodium Azide
1% - Bovine Serum Albumin