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HRV3C Protease

GWB-15AECB

45-005-155001

1 mg (1000 units)
$255.00
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Additional Information

Name HRV3C Protease
Description of Target The recombinant Turbo3C Protease is a restriction grade HRV3C protease that has robust activity at 4°C and high specific activity and great stability. It does not require any special buffer for its activity and is compatible with buffers most suitable for target proteins.
Peptide Sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro
Tag N-terminal His Tag or GST tag
Product Format Liquid 25mM Tris-HCl (pH 8.0), 50mM NaCl, 1mM TCEP and 50% glycerol. The Storage Buffer is compatible with Ni-chelating resin and GSH resin for on-column digestion.
Molecular Weight 47 kDa
Datasheets / Downloads GWB-15AECB Datasheet
Purification Affinity chromatography
Specificity Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly.
Biological Activity 1 ug has at least 1 unit activity by the conventional defintion: 1 unit of GWB-15AECB cleaves >95% of 100 ug of a control target protein at 4°C in 16 hr.
Concentration 2 mg/ml
Applications It is recommended to use GWB-15AECB at a protease to target protein ratio of 1:100 (w/w) or 1 unit of target protein concentration at 1-2 mg/ml. In most cases, target proteins are completely cleaved with a protease to target protein ratio of 1:50 to 1:400 or 1 unit GWB-15AECB to 50-400 ug of target protein. The efficiency of cleavage may vary due to the sequences around the cleavage site, the conformation and the solubility of the target protein. Due to its high specificity, more GWB-15AECB (at 1:10 ratio), or longer cleavage time (over a weekend), or at higher temperature (37°C) can be used to achieve high cleavage efficiency without non-specific cleavage of target proteins.
Removal of Protease after Cleavage:
After cleavage of target protein, GWB-15AECB can be easily removed in a single step together with the tags from the cleavage reaction by affinity chromatography on a Ni-chelating resin for His-tagged target protein or GSH resin for GST-tagged target protein.
Reconstitution and Storage Store product at -20°C. Protease is stable at room temperature for at least two weeks without loss of any activity. It retains full activity in Storage Buffer after incubation at 37°C for one week.
Additional Information Non-specific cleavage of the target protein is not observed under the same condition at 1:10 (w/w) ratio of GWB-15AECB to control target protein. Extended incubation of target proteins with GWB-15AECB for several days does not show any non-specific cleavage.