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SLIDE-A-LYZER DIALYSIS CASSETTE Lab Equipment (GWB-Q00430)

This exact product is now provided by Aviva Systems Biology.
Please visit OOSA11305 to review further and for ordering information.


GWB-Q00430

1 Unit
$99.00

Description

Description: The dialysis cassette acts as a convenient way of dialysing samples. Removal of low molecular weight contaminants, buffer exchange, desalting and concentration can be accomplished with this device. The cassette features a hermetically sealed chamber to maintain the highest possible sample integrity. It has been manufactured under clean room conditions to ensure that the unit is free of contaminants. Sample introduction and removal is easily accomplished by penetrating the silicone gasket with a hypodermic needle attached to a syringe. The silicone imparts re-sealability to the needle puncture, ensuring that no sample is lost from the cassette during dialysis.

Additional Information

Name SLIDE-A-LYZER DIALYSIS CASSETTE Lab Equipment (GWB-Q00430)
Related Product Names SLIDE-A-LYZER DIALYSIS CASSETTE Lab Equipment, SLIDE-A-LYZER DIALYSIS CASSETTE
Product Format Kit; Lab Equipment
Molecular Weight MW cut-off 10kDa
Application Functional Assay
Format Kit
Stability 2 years from date of despatch.
Reconstitution and Storage Store at 18C to 25C.
Datasheets/Manuals Printable datasheet for GWB-Q00430
Storage Store at +18oC to +25oC.
Lead Time Domestic: within 2-3 weeks delivery | International: 2-3 weeks
Intended Use Research Use Only
Additional Information Typical sample volume range: 0.5ml - 3.0ml
Sample volume range for (NH4)2SO4 containing samples: 0.5ml - 2.5ml
:: Instructions For Use:

1. Remove the Dialysis Cassette from its protective pouch. Note: In order to prevent contamination, handle the cassette by the plastic frame, do not touch the membrane with ungloved hands. The cassette can be slipped into the groove of a buoy or a bench top stand.

2. Attach the hub of the hypodermic needle to the Luer-LokTM fitting of the syringe by firmly screwing it into place. Note: Do not remove the plastic sheath over the needle until you are ready to fill the syringe with your sample. Use caution when working with the hypodermic needle to avoid injury. The Dialysis Cassette is designed to be used with 18 gauge bevelled, 1 inch needles (21 gauge 1 inch needles can also be used).

3. Remove the protective sheath from the hypodermic needle and fill the syringe with your sample by first immersing the needle in your sample and then slowly drawing back on the syringe piston.

4. Remove the cassette from the buoy or stand and fill with 0.5-3.0ml of sample (only up to 2.5ml for samples containing (NH4)2SO4). After penetrating the silicone gasket with the needle through one of the syringe ports at the corner of the cassette. Mark the corner of the cassette with a permanent marker. Note: The bevelled portion of the needle should penetrate the silicone gaskets to a minimal extent. Overextending the needle into the cavity can potentially puncture the membrane. If you have a sample with high protein concentration (such as 10mg/ml), fill the cassette slowly to avoid foaming.

5. With the syringe needle still inserted in the cassette cavity draw up on the syringe piston to remove air from the cassette cavity. This will compress the membrane windows so that the sample solution contacts the greatest window surface area.

6. Remove the syringe needle from the cassette while retaining the air in the syringe. The silicone gasket will reseal and the membrane cavity will have no (or minimal) air in direct contact with the sample.

7. Slip the cassette into the stand and float this assembly in the dialysis solution of choice.

8. To remove the sample after dialysis, fill the syringe with 4ml of air and penetrate the silicone gasket with the needle through another of the syringe guide ports at the bottom of the cassette. Discharge the air into the cassette cavity. Note: Do not penetrate the guide ports more than once, since this may lead to coring of the

silicone and subsequent loss of the sample.

9. Slowly draw back on the syringe piston to capture the dialyzed sample within the syringe barrel. Remove the syringe needle from the cassette.