Proteases

We have 2 Proteases

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    1. $255.00
      GenWay ID: GWB-15AECB
      Legacy ID: 45-005-155001
      Item Type: Reagents, Proteases.
      Application(s): It is recommended to use GWB-15AECB at a protease to target protein ratio of 1:100 (w/w) or 1 unit of target protein concentration at 1-2 mg/ml. In most cases, target proteins are completely cleaved with a protease to target protein ratio of 1:50 to 1:400 or 1 unit GWB-15AECB to 50-400 ug of target protein. The efficiency of cleavage may vary due to the sequences around the cleavage site, the conformation and the solubility of the target protein. Due to its high specificity, more GWB-15AECB (at 1:10 ratio), or longer cleavage time (over a weekend), or at higher temperature (37°C) can be used to achieve high cleavage efficiency without non-specific cleavage of target proteins.
      Removal of Protease after Cleavage:
      After cleavage of target protein, GWB-15AECB can be easily removed in a single step together with the tags from the cleavage reaction by affinity chromatography on a Ni-chelating resin for His-tagged target protein or GSH resin for GST-tagged target protein.

    2. Varies by Size
      GenWay ID: GWB-1F45A6
      Legacy ID: 45-005-155002
      Item Type: Reagents, Proteases.

    2 Item(s)

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