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TMB One Component HRP Microwell Substrate



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TMB One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. Unreacted substrate should be colorless or very light yellow in appearance. When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained. The reaction can be stopped using appropriate stop solution, producing a soluble yellow or soluble blue reaction product, depending upon the stop reagent used, which is stable for at least 1 hour. TMB One Component HRP Microwell Substrate is not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Additional Information

Name TMB One Component HRP Microwell Substrate
Related Product Names TMB One Component HRP Microwell Substrate
Application Info Dilution of the substrate is not recommended. The intensity of the reaction can be reduced by further dilution of the antibodies/conjugates used in the assay or to shorten the incubation time.
Reconstitution and Storage Allow the substrate to equilibrate at room temperature (~25°C) prior to use. Product should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule. Please note that TMB substrates are shipped to customers at ambient temperatures. Extensive stability studies have shown that prolonged storage at ambient temperature will not affect the product quality or efficacy.
Datasheets/Manuals Printable datasheet for GWB-374D5C
Intended Use Research Use Only