TMB Slow Kinetic One Component HRP Microwell Substrate

GWB-12B551

45-053-150017

$0.00
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Description

TMB Slow Kinetic One Component HRP Microwell Substrate contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. Unreacted substrate should be colorless or very light yellow in appearance. When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained. The reaction can be stopped using appropriate stop solution, producing a soluble yellow or soluble blue reaction product, depending upon the stop reagent used, which is stable for at least 1 hour. TMB Slow Kinetic One Component HRP Microwell Substrate is not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.

Additional Information

Name TMB Slow Kinetic One Component HRP Microwell Substrate
Related Product Names TMB Slow Kinetic One Component HRP Microwell Substrate
Application Data 1. Test: pH
Specification: 3.55±0.20
Result: 3.47
2. Test:Absorbance at 285nm
Specification: 0.450±0.100
Result: 0.417
3. Test: Absorbance at 650nm
Specification: ≤0.020
Result: 0.000
4. Test: Appearance
Specification: Colorless to very light yellow
Result: Conforms.
Reconstitution and Storage TMB Substrate is shipped at ambient temperature. Product should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule. Allow the substrate to equilibrate at room temperature (~25°C) prior to use.
Datasheets/Manuals Printable datasheet for GWB-12B551
Intended Use Research Use Only