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RPS6KB1 ELISA Kit (Human) (GWB-KBBXG3)

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GWB-KBBXG3

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Additional Information

Name RPS6KB1 ELISA Kit (Human) (GWB-KBBXG3)
Description of Target Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 an
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.058 ng/mL
Sample Type tissue homogenates and other biological fluids.
Kit Detection Colorimetric, OD 450 nm
Gene Symbol RPS6KB1
Gene id 6198
Alias Symbols 70 kDa ribosomal protein S6 kinase 1, p70(S6K)-alpha, p70-alpha, p70 ribosomal S6 kinase alpha, p70-S6K, P70S6K1, p70-S6K 1, p70 S6KA, p70 S6K-alpha, p70 S6 kinase alpha, PS6K, Ribosomal protein S6 kinase beta-1, Ribosomal protein S6 kinase I, S6K, S6K1,
Swissprot ID P23443
Protein Name ribosomal protein S6 kinase beta-1
Kit Principle Genway Biotech RPS6KB1 ELISA Kit (Human) (GWB-KBBXG3) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RPS6KB1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RPS6KB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RPS6KB1 captured in the well.
Kit Component
ComponentAmount
Anti-Prkar2b Microplate96 Wells (12 x 8 Well Strips)
Prkar2b Lyophilized Standard2 x 10 ng
100X Biotinylated Prkar2b Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_001258971
Nucleotide Accession Num NM_001272042
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBXG3
Species Reactivity Human