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SDPR ELISA Kit (Human) (GWB-KBBUC7)

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GWB-KBBUC7

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Additional Information

Name SDPR ELISA Kit (Human) (GWB-KBBUC7)
Description of Target May play a role in targeting PRKCA to caveolae.
Kit Range 0.156 - 10 ng/mL
Sensitivity < 0.054 ng/mL
Sample Type tissue homogenates, cell lysates and other biological fluids
Kit Detection Colorimetric, OD 450 nm
Gene Symbol SDPR
Gene id 8436
Alias Symbols CAVIN2, cavin-2, Cavin-2, Phosphatidylserine-binding protein, PS-p68, SDR, Serum deprivation-response protein
Swissprot ID O95810
Protein Name Serum deprivation-response protein
Kit Principle Genway Biotech SDPR ELISA Kit (Human) (GWB-KBBUC7) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SDPR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SDPR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SDPR captured in the well.
Kit Component
ComponentAmount
Anti-SDPR Microplate96 Wells (12 x 8 Well Strips)
SDPR Lyophilized Standard2 x 10 ng
100X Biotinylated SDPR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Protein Accession Num NP_004648
Nucleotide Accession Num NM_004657
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Reconstitution and Storage Store at 2 - 8C. Do not use the kit beyond the expiration date.
Kit Duration ~ 3 Hours
Datasheets/Manuals Printable datasheet for GWB-KBBUC7
Species Reactivity Human