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Peroxidase Conjugated Affinity Purified Anti-CHICKEN IgG (H&L) (RABBIT)



1.5 mg
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Antigen: IgG

Fraction: Affinity Purified IgG

Additional Information

Name Peroxidase Conjugated Affinity Purified Anti-CHICKEN IgG (H&L) (RABBIT)
Related Product Names Peroxidase Conjugated Affinity Purified anti-Chicken IgG [H&L] [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-CHICKEN IgG (H&L) (RABBIT)IGHG1
Swissprot ID P01857
Host Rabbit
Isotype IgG
Conjugation Peroxidase (Horseradish) - HRP
Immunogen Chicken IgG whole molecule
Product Format Lyophilized
Purification Anti-Chicken IgG/IgY Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and Chicken IgG. Some light chain cross reactivity may be observed against chicken immunoglobulins. Known Cross Reactivity:
Chicken IgY / IgG
Applications WB, ELISA, IHC
Application Info All assays should be optimized by the user. Recommended dilutions may be listed below.
ELISA: 1:75,000-1:150,000
WB: 1:2,500 - 1:15,000
IHC: 1:1,000 - 1:5,000
Format Lyophilized
Reconstitution and Storage Store vial at 4 C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
Reactivity Chicken IgG
Storage 4C
Datasheets/Manuals Printable datasheet for GWB-35B4C7
Intended Use Research Use Only
Additional Information Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Color: Substrate dependent
Conjugation Reference: Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
:: Preservative: 0.01% (w/v) Gentamicin Sulfate (Do NOT add Sodium Azide!)
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free