Peroxidase Conjugated Affinity Purified Anti-GOAT IgG F(ab')2 (RABBIT)

GWB-25D7AD

25-732-294063

2 mg
$260.00
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Description

Antigen: This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 110 - 150 is suggested. Acetone fixation of the antigen source is recommended prior to staining. ViroStat also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#4400) and its MOUNTING MEDIUM (Cat.#4300) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing ViroStat's enzyme-antibody conjugates specific to FITC (Cat.#s 3104 & 3105). Recommened short term (

Fraction: This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 110 - 150 is suggested. Acetone fixation of the antigen source is recommended prior to staining. ViroStat also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#4400) and its MOUNTING MEDIUM (Cat.#4300) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing ViroStat's enzyme-antibody conjugates specific to FITC (Cat.#s 3104 & 3105). Recommened short term (

Additional Information

Name Peroxidase Conjugated Affinity Purified Anti-GOAT IgG F(ab')2 (RABBIT)
Related Product Names Peroxidase Conjugated Affinity Purified anti-Goat IgG F(ab'’)2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-GOAT IgG F(ab')2 (RABBIT)IGHG1
Alias Symbols HRP
Swissprot ID P01857
Clone Polyclonal
Immunogen Goat IgG F(ab')2 fragment
Product Format Lyophilized
Molecular Weight 40000
Applications WB, ELISA, IHC
Application Info ELISA: 1:10,000 - 1:50,000 WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500
Format Lyophilized
Reconstitution and Storage Store vial at 4 C prior to restoration.     Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below.   Avoid cycles of freezing and thawing.   Centrifuge product if not completely clear after standing at room temperature.   This product is stable for several weeks at 4 C as an undiluted liquid.   Dilute only prior to immediate use.     Expiration date is one (1) year from date of restoration.
Reactivity Goat IgG F(ab')2
Storage 4C
Datasheets/Manuals Printable datasheet for GWB-25D7AD
Intended Use Research Use Only
Additional Information Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Color: Substrate dependent
Conjugation Reference: Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
:: Label: Peroxidase (Horseradish)
Preservative: 0.01% (w/v) Gentamicin Sulfate (Do NOT add Sodium Azide!)
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)  - Immunoglobulin and Protease free