Peroxidase Conjugated Affinity Purified Anti-GOAT IgG F(ab')2 (RABBIT)

GWB-25D7AD

25-732-294063

2 mg
$260.00
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Description

Antigen: This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 110 - 150 is suggested. Acetone fixation of the antigen source is recommended prior to staining. ViroStat also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#4400) and its MOUNTING MEDIUM (Cat.#4300) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing ViroStat's enzyme-antibody conjugates specific to FITC (Cat.#s 3104 & 3105). Recommened short term (

Fraction: This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 110 - 150 is suggested. Acetone fixation of the antigen source is recommended prior to staining. ViroStat also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#4400) and its MOUNTING MEDIUM (Cat.#4300) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing ViroStat's enzyme-antibody conjugates specific to FITC (Cat.#s 3104 & 3105). Recommened short term (

Additional Information

Name Peroxidase Conjugated Affinity Purified Anti-GOAT IgG F(ab')2 (RABBIT)
Related Product Names Peroxidase Conjugated Affinity Purified anti-Goat IgG F(ab'’)2 [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-GOAT IgG F(ab')2 (RABBIT)IGHG1
Alias Symbols HRP
Clone Polyclonal
Immunogen Goat IgG F(ab')2 fragment
Product Format Lyophilized
Molecular Weight 40000
Datasheets / Downloads GWB-25D7AD Datasheet
Swiss Prot Number P01857
Applications WB, ELISA, IHC
Application Info ELISA: 1:10,000 - 1:50,000 WB: 1:1,000 - 1:10,000 IHC: 1:500 - 1:2,500
Format Lyophilized
Reconstitution and Storage Store vial at 4 C prior to restoration.     Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below.   Avoid cycles of freezing and thawing.   Centrifuge product if not completely clear after standing at room temperature.   This product is stable for several weeks at 4 C as an undiluted liquid.   Dilute only prior to immediate use.     Expiration date is one (1) year from date of restoration.
Reactivity Goat IgG F(ab')2
Storage 4C
Intended Use Research Use Only
Additional Information Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Color: Substrate dependent
Conjugation Reference: Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.
:: Label: Peroxidase (Horseradish)
Preservative: 0.01% (w/v) Gentamicin Sulfate (Do NOT add Sodium Azide!)
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA)  - Immunoglobulin and Protease free