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Peroxidase Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT) (Min X Human Serum Proteins)



1 mg
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Antigen: Goat IgG MX Human (Rb)

Immunogen: Goat IgG whole molecule

Family: Affinity Purified

Fraction: Affinity Purified IgG

Conjugation: Peroxidase

Conjugation Reference: Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.

Application: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Recommended Dilution: This product has been assayed against 1.0 ?g of Goat IgG in a standard captureELISA using ABTS (2,2\'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30minutes at room temperature. A working dilution of 1:14,000 to 1:60,000 of the reconstitution concentration issuggested for this product. Optimal titers for other applications should be determined by the researcher.

ELISA: 1:10,000 - 1:50,000
IHC: 1:500 - 1:2,500
WB: 1:1,000 - 1:5,000

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Buffer: 0.02 M Postassium Phosphate, 0.15M Sodium Chloride, pH 7.2

Physical State: Lyophilized

Purity and Specificity: This product was prepared from monospecific antiserum by immunoaffinitychromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove anyunwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Goat IgG and Goat Serum. No reaction was observed against Human SerumProteins.

Antibody Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Label: Horseradish Peroxidase (HRP)

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free

Subcellular Location: Secreted.

Disease: Chromosomal aberrations involving IGHG1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.

Miscellaneous: Nie has the G1M(17) allotypic marker, 97-K, and the G1M(1) markers, 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

Miscellaneous: Nie also differs in the amidation states of 35, 116, 198, 269 and 272.

Miscellaneous: EU also differs in the amidation states of residues 155, 166, 177, 195, 198, 269, and 272 and in the order of residues 268-272.

Miscellaneous: KOL also differs in the amidation states of residues 198, 267 and 272.

Additional Information

Name Peroxidase Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT) (Min X Human Serum Proteins)
Related Product Names Peroxidase Conjugated Affinity Purified anti-Goat IgG [H&L] [Rabbit] Minimum Cross reactivity to Human Serum Proteins; N/A Peroxidase Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT) (Min X Human Serum Proteins)IGHG1
Swissprot ID P01857
Molecular Weight 36106
Purity Affinity Purified
Source Rabbit
Applications WB, ELISA, IHC
Format Lyophilized
Reactivity Goat IgG
Storage 4C
Datasheets/Manuals Printable datasheet for GWB-3D9C97
Intended Use Research Use Only