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Rhodamine Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)



2 mg
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Antigen: IgG

Immunogen: Goat IgG whole molecule

Family: Affinity Purified

Fraction: Affinity Purified IgG

Purity Note: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.

Conjugation: Rhodamine

Conjugation Reference: J.A. Titus, et al. J. Immunol. Methods 50; 193, 1982.

Application: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Antibody Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)

Assay Dilutions: All assays should be optimized by the user. Recommended dilutions (if any) may be listed below.

FLISA: 1:10,000 - 1:50,000   IF Microscopy: 1:1,000 - 1:5,000

Buffer: 0.02 M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2

Label: Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons)

Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free

Subcellular Location: Secreted.

Disease: Chromosomal aberrations involving IGHG1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with CCND1; translocation t(4;14)(p16.3;q32.3) with FGFR3; translocation t(6;14)(p25;q32) with IRF4.

Miscellaneous: Nie has the G1M(17) allotypic marker, 97-K, and the G1M(1) markers, 239-D and 241-L. KOL and EU sequences have the G1M(3) marker and the G1M (non-1) markers.

Miscellaneous: Nie also differs in the amidation states of 35, 116, 198, 269 and 272.

Miscellaneous: EU also differs in the amidation states of residues 155, 166, 177, 195, 198, 269, and 272 and in the order of residues 268-272.

Miscellaneous: KOL also differs in the amidation states of residues 198, 267 and 272.

Additional Information

Name Rhodamine Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)
Related Product Names Rhodamine Conjugated Affinity Purified anti-Goat IgG [H&L] [Rabbit]; N/A Rhodamine Conjugated Affinity Purified Anti-GOAT IgG (H&L) (RABBIT)IGHG1
Swissprot ID P01857
Molecular Weight 36106
Purity Affinity Purified
Source Rabbit
Applications WB, ELISA, IHC, Fluorescence Microscopy, FACS
Format Lyophilized
Reactivity Goat IgG
Storage 4C
Datasheets/Manuals Printable datasheet for GWB-5EBB84
Intended Use Research Use Only