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Affinity Purified Anti-HUMAN IgG F(c) (GOAT) (Min X MOUSE Serum Proteins)



1 mg
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Additional Information

Name Affinity Purified Anti-HUMAN IgG F(c) (GOAT) (Min X MOUSE Serum Proteins)
Alias Symbols Goat Anti Human IgG Fc Antibody Pre-Adsorbed, Goat Anti-Human IgG F(c) Antibody, Goat Anti Human IgG Fc Fragment Antibody
Swissprot ID P01857
Species Human
Description of Target Anti-Human IgG F(c) generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH. Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Clone Polyclonal
Host Goat
Isotype IgG
Immunogen Human IgG F(c) fragment
Product Format Liquid. 0.02 M Postassium Phosphate, 0.15 M Sodium Chloride (pH 7.2) with 0.01% (w/v) Sodium azide.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against Human IgG F(ab) or Mouse Serum Proteins. Specificity was confirmed by ELISA minimal cross reactivity against Mouse IgG.
Concentration 1 mg/ml
Applications Anti-Human IgG F(c) antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. All assays should be optimized by the user.
Application Info ELISA: 1:15,000 - 1:60,000 WB: 1:1,000 - 1:5,000 IHC: 1:1,000 - 1:3,000
Stability Expiration date is one (1) year from date of opening product.
Reconstitution and Storage Store vial at 4°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Datasheets/Manuals Printable datasheet for GWB-20A705